PMID- 15865979
OWN - NLM
STAT- MEDLINE
DCOM- 20050808
LR  - 20101118
IS  - 0076-6879 (Print)
IS  - 0076-6879 (Linking)
VI  - 395
DP  - 2005
TI  - Preparation of samples for comparative studies of plant chromosomes using in situ 
      hybridization methods.
PG  - 443-60
AB  - The development of fluorescence in situ hybridization (FISH) has led to the 
      advancement of chromosome studies not only for physical mapping and genome 
      analyses but also as a tool for evolutionary studies. Isolated repetitive DNA 
      sequences have been useful cytological markers, but large-insert genomic 
      libraries (e.g., bacterial artificial chromosomes [BACs], yeast artificial 
      chromosomes [YACs], and cosmids) are being increasingly used to serve as probes 
      for large segments of DNA across related genomes. Although FISH is usually 
      applied to metaphase chromosomes, fiber FISH, a variation of FISH using extended 
      DNA fibers, is now used to measure loci at the resolution of a few kilobases to 
      compare orthologous genome segments across related genomes. A generalized set of 
      protocols for chromosome preparation, FISH, and fiber FISH are presented; 
      however, it is often necessary to experiment with techniques for different plant 
      taxa for successful molecular cytogenetic studies.
FAU - Walling, Jason G
AU  - Walling JG
AD  - Department of Agronomy, Purdue University, West Lafayette, Indiana 47907, USA.
FAU - Pires, J Chris
AU  - Pires JC
FAU - Jackson, Scott A
AU  - Jackson SA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Biological Evolution
MH  - Cell Fractionation/methods
MH  - Chromosomes, Plant/*genetics
MH  - DNA, Plant/genetics/isolation & purification
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Meiosis/genetics
MH  - Mitosis/genetics
MH  - Molecular Probe Techniques
MH  - Nucleic Acid Denaturation
MH  - Plant Roots/genetics
MH  - Plants/*genetics
EDAT- 2005/05/04 09:00
MHDA- 2005/08/09 09:00
CRDT- 2005/05/04 09:00
PHST- 2005/05/04 09:00 [pubmed]
PHST- 2005/08/09 09:00 [medline]
PHST- 2005/05/04 09:00 [entrez]
AID - S0076687905950246 [pii]
AID - 10.1016/S0076-6879(05)95024-6 [doi]
PST - ppublish
SO  - Methods Enzymol. 2005;395:443-60. doi: 10.1016/S0076-6879(05)95024-6.