PMID- 15866225
OWN - NLM
STAT- MEDLINE
DCOM- 20050722
LR  - 20131121
IS  - 0955-2863 (Print)
IS  - 0955-2863 (Linking)
VI  - 16
IP  - 5
DP  - 2005 May
TI  - Effect of postexercise carbohydrate supplementation on glucose uptake-associated 
      gene expression in the human skeletal muscle.
PG  - 267-71
AB  - We previously found that the exercise-induced elevation in GLUT4 mRNA of rat 
      muscle can be rapidly down-regulated when glucose is given immediately following 
      exercise. The purpose of this study was to determine the effect of postexercise 
      carbohydrate diet on GLUT4 and hexokinase (HK) II mRNA levels in the human 
      skeletal muscle. Eight untrained male subjects (age, 20.7+/-3.1 years) exercised 
      for 60 min on a cycle ergometer at a 70-75% maximal oxygen consumption. The 
      postexercise dietary treatment was performed in a crossover design. Immediately 
      after the exercise, a diet with 70% carbohydrate content (1 g per kilogram of 
      body weight; 356+/-19.8 kcal) was given to half of the subjects (eaten in 10 min) 
      followed by a 3-h recovery, while the control subjects remained unfed for 3 h. 
      Biopsies were performed on the deep portion of the vastus lateralis muscle of all 
      subjects immediately after the exercise and 3 h after the carbohydrate ingestion. 
      Blood glucose and serum insulin concentrations were measured every 30 min for 3 
      h. At the end of the 3-h recovery, blood glucose and serum insulin levels were 
      not different from control levels, indicating that the oral carbohydrate was 
      mostly disposed in the body within 3 h. In addition, GLUT4 and HK II mRNA levels 
      were significantly lowered in the exercised human skeletal muscle in subjects 
      receiving the carbohydrate diet. In conclusion, the present study demonstrates 
      that GLUT4 mRNA and HK II mRNA in the exercised human skeletal muscle were 
      significantly lowered by a high-carbohydrate diet.
FAU - Cheng, I-Shiung
AU  - Cheng IS
AD  - Department of Nutrition and Food Sciences, Fu-Jen Catholic University, Taipei, 
      Taiwan 242, ROC.
FAU - Lee, Ning-Yuean
AU  - Lee NY
FAU - Liu, Kai-Li
AU  - Liu KL
FAU - Liao, Su-Fen
AU  - Liao SF
FAU - Huang, Chiung-Hsueh
AU  - Huang CH
FAU - Kuo, Chia-Hua
AU  - Kuo CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Nutr Biochem
JT  - The Journal of nutritional biochemistry
JID - 9010081
RN  - 0 (Blood Glucose)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Monosaccharide Transport Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (SLC2A4 protein, human)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Blood Glucose/metabolism
MH  - Dietary Carbohydrates/*pharmacology
MH  - Down-Regulation
MH  - Exercise/*physiology
MH  - Gene Expression Regulation/*drug effects
MH  - Glucose/*metabolism
MH  - Glucose Transporter Type 4
MH  - Hexokinase/biosynthesis
MH  - Humans
MH  - Male
MH  - Monosaccharide Transport Proteins/*biosynthesis
MH  - Muscle Proteins/*biosynthesis
MH  - Muscle, Skeletal/drug effects/*metabolism
EDAT- 2005/05/04 09:00
MHDA- 2005/07/23 09:00
CRDT- 2005/05/04 09:00
PHST- 2005/05/04 09:00 [pubmed]
PHST- 2005/07/23 09:00 [medline]
PHST- 2005/05/04 09:00 [entrez]
AID - S0955-2863(04)00260-8 [pii]
AID - 10.1016/j.jnutbio.2004.12.006 [doi]
PST - ppublish
SO  - J Nutr Biochem. 2005 May;16(5):267-71. doi: 10.1016/j.jnutbio.2004.12.006.