PMID- 15866642
OWN - NLM
STAT- MEDLINE
DCOM- 20050926
LR  - 20151119
IS  - 0041-1345 (Print)
IS  - 0041-1345 (Linking)
VI  - 37
IP  - 3
DP  - 2005 Apr
TI  - Markers of fibrosis in early biopsies of renal transplants.
PG  - 1468-70
AB  - Many factors are involved in the development of chronic allograft nephropathy 
      (CAN). Extracellular matrix turnover depends on the balance between fibrogenic 
      and antifibrogenic cytokines. The aim of our study was to analyze the presence of 
      transforming growth factor beta-1 (TGF-beta1), matrix metalloproteinase-2 
      (MMP-2), and mast cells in 53 early transplant biopsies using immunochemistry 
      with specific monoclonal antibodies. We divided the patients into two groups 
      depending on graft evolution (lost due to CAN versus functioning), renal 
      function, presence of proteinuria, and graft survival. There were no differences 
      in the demographic or immunological data. Renal function was worse and 
      proteinuria greater among the group with CAN. The presence of mast cells was 
      similar in both groups, but TGF-beta1 was expressed more and MMP-2 less in the 
      CAN group. We observed a negative correlation between donor age and mast cells, 
      and a positive correlation between TGF-beta1 and MMP-2. Grafts from younger 
      donors showed better renal function, less proteinuria, greater graft survival, 
      and less frequent development of CAN. According to our experience, cytokines 
      involved in matrix turnover are expressed in early stages, correlating with donor 
      age. The expressions of TGF-beta1 and MMP-2 seem to be important for the 
      development of fibrosis in CAN.
FAU - Palomar, R
AU  - Palomar R
AD  - Nephrology Department, Marques de Valdecilla Hospital, University of Cantabria, 
      Santander, Spain. mpalomarf@wanadoo.es
FAU - Mayorga, M
AU  - Mayorga M
FAU - Ruiz, J C
AU  - Ruiz JC
FAU - Cuevas, J
AU  - Cuevas J
FAU - Rodrigo, E
AU  - Rodrigo E
FAU - Cotorruelo, J G
AU  - Cotorruelo JG
FAU - Val-Bernal, J F
AU  - Val-Bernal JF
FAU - Arias, M
AU  - Arias M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (Biomarkers)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Adult
MH  - Biomarkers/blood
MH  - Biopsy
MH  - Female
MH  - Fibrosis
MH  - Histocompatibility Testing
MH  - Humans
MH  - Kidney Glomerulus/pathology
MH  - Kidney Transplantation/*pathology
MH  - Kidney Tubules/pathology
MH  - Male
MH  - Mast Cells/pathology
MH  - Matrix Metalloproteinase 2/metabolism
MH  - Middle Aged
MH  - Renal Circulation
MH  - Transforming Growth Factor beta/metabolism
MH  - Transforming Growth Factor beta1
EDAT- 2005/05/04 09:00
MHDA- 2005/09/27 09:00
CRDT- 2005/05/04 09:00
PHST- 2005/05/04 09:00 [pubmed]
PHST- 2005/09/27 09:00 [medline]
PHST- 2005/05/04 09:00 [entrez]
AID - S0041-1345(05)00167-3 [pii]
AID - 10.1016/j.transproceed.2005.02.051 [doi]
PST - ppublish
SO  - Transplant Proc. 2005 Apr;37(3):1468-70. doi: 10.1016/j.transproceed.2005.02.051.