PMID- 15866891
OWN - NLM
STAT- MEDLINE
DCOM- 20050811
LR  - 20230508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 169
IP  - 3
DP  - 2005 May 9
TI  - STIM1, an essential and conserved component of store-operated Ca2+ channel 
      function.
PG  - 435-45
AB  - Store-operated Ca2+ (SOC) channels regulate many cellular processes, but the 
      underlying molecular components are not well defined. Using an RNA interference 
      (RNAi)-based screen to identify genes that alter thapsigargin (TG)-dependent Ca2+ 
      entry, we discovered a required and conserved role of Stim in SOC influx. 
      RNAi-mediated knockdown of Stim in Drosophila S2 cells significantly reduced 
      TG-dependent Ca2+ entry. Patch-clamp recording revealed nearly complete 
      suppression of the Drosophila Ca2+ release-activated Ca2+ (CRAC) current that has 
      biophysical characteristics similar to CRAC current in human T cells. Similarly, 
      knockdown of the human homologue STIM1 significantly reduced CRAC channel 
      activity in Jurkat T cells. RNAi-mediated knockdown of STIM1 inhibited TG- or 
      agonist-dependent Ca2+ entry in HEK293 or SH-SY5Y cells. Conversely, 
      overexpression of STIM1 in HEK293 cells modestly enhanced TG-induced Ca2+ entry. 
      We propose that STIM1, a ubiquitously expressed protein that is conserved from 
      Drosophila to mammalian cells, plays an essential role in SOC influx and may be a 
      common component of SOC and CRAC channels.
FAU - Roos, Jack
AU  - Roos J
AD  - Torrey Pines Therapeutics, Inc., La Jolla, CA 92037, USA.
FAU - DiGregorio, Paul J
AU  - DiGregorio PJ
FAU - Yeromin, Andriy V
AU  - Yeromin AV
FAU - Ohlsen, Kari
AU  - Ohlsen K
FAU - Lioudyno, Maria
AU  - Lioudyno M
FAU - Zhang, Shenyuan
AU  - Zhang S
FAU - Safrina, Olga
AU  - Safrina O
FAU - Kozak, J Ashot
AU  - Kozak JA
FAU - Wagner, Steven L
AU  - Wagner SL
FAU - Cahalan, Michael D
AU  - Cahalan MD
FAU - Velicelebi, Gonul
AU  - Velicelebi G
FAU - Stauderman, Kenneth A
AU  - Stauderman KA
LA  - eng
GR  - R01 NS014609/NS/NINDS NIH HHS/United States
GR  - R37 NS014609/NS/NINDS NIH HHS/United States
GR  - R56 NS014609/NS/NINDS NIH HHS/United States
GR  - NS14609/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20050502
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Calcium Channels)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Membrane Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (STIM1 protein, human)
RN  - 0 (Stim protein, Drosophila)
RN  - 0 (Stromal Interaction Molecule 1)
RN  - 67526-95-8 (Thapsigargin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
CIN - J Cell Biol. 2005 May 9;169(3):381-2. PMID: 15866892
MH  - Animals
MH  - Calcium/*metabolism
MH  - Calcium Channels/*metabolism
MH  - Calcium Signaling/drug effects/*physiology
MH  - Cell Line
MH  - Cell Membrane/*metabolism
MH  - Conserved Sequence/physiology
MH  - Drosophila
MH  - Drosophila Proteins/genetics/isolation & purification/*metabolism
MH  - Enzyme Inhibitors/pharmacology
MH  - Evolution, Molecular
MH  - Humans
MH  - Jurkat Cells
MH  - Membrane Proteins/genetics/isolation & purification/*metabolism
MH  - Neoplasm Proteins/genetics/*metabolism
MH  - Patch-Clamp Techniques
MH  - RNA Interference
MH  - Stromal Interaction Molecule 1
MH  - Thapsigargin/pharmacology
PMC - PMC2171946
EDAT- 2005/05/04 09:00
MHDA- 2005/08/12 09:00
PMCR- 2005/11/09
CRDT- 2005/05/04 09:00
PHST- 2005/05/04 09:00 [pubmed]
PHST- 2005/08/12 09:00 [medline]
PHST- 2005/05/04 09:00 [entrez]
PHST- 2005/11/09 00:00 [pmc-release]
AID - jcb.200502019 [pii]
AID - 200502019 [pii]
AID - 10.1083/jcb.200502019 [doi]
PST - ppublish
SO  - J Cell Biol. 2005 May 9;169(3):435-45. doi: 10.1083/jcb.200502019. Epub 2005 May 
      2.