PMID- 15869916
OWN - NLM
STAT- MEDLINE
DCOM- 20051122
LR  - 20131121
IS  - 8756-3282 (Print)
IS  - 1873-2763 (Linking)
VI  - 36
IP  - 6
DP  - 2005 Jun
TI  - Fluid flow stimulates expression of osteopontin and bone sialoprotein by bone 
      marrow stromal cells in a temporally dependent manner.
PG  - 1047-55
AB  - Bone marrow stromal cells (BMSCs) are multipotent progenitor cells with a 
      capacity to form bone tissue in vivo, and to differentiate into the osteoblastic 
      lineage in vitro. Drawing on evidence that bone is mechanosensitive and 
      mechanical stimuli are anabolic, we postulate that proliferation and osteoblastic 
      differentiation of BMSCs may be stimulated by mechanical forces. In this study, 
      BMSCs cultured in the presence of osteogenic factors (dexamethasone, 
      beta-glycerophosphate, and ascorbate) were stimulated repeatedly (every second 
      day) with shearing flow (1.6 dyn/cm(2)) for 5, 30, or 120 min, and assayed for 
      systematic changes in cell number and phenotypic markers of osteoblastic 
      differentiation. Cells exposed to fluid flow on days 2 and 4 after the addition 
      of osteogenic factors and assayed at day 6 exhibited a modest decrease in cell 
      number and increase in normalized alkaline phosphatase activity, suggesting the 
      detachment of a non-osteogenic subpopulation. Cells exposed to fluid flow on days 
      6, 8, 10, and 12 and assayed at day 20 demonstrated maximal expression of 
      osteopontin and bone sialoprotein mRNA with 30 min duration of flow. 
      Concurrently, at day 20 expression of the adipogenic marker, lipoprotein lipase, 
      was minimal with a 120-min duration of flow. These results indicate that repeated 
      application of shear stress stimulates late phenotypic markers of osteoblastic 
      differentiation of BMSCs in a manner that depends on the duration of stimulus. 
      Finally, accumulation of prostaglandin E(2) in culture medium in response to 
      shearing flow systematically decreased with repeated exposure to 30 and 120 min 
      of shear stress (from day 6 to day 12), suggesting an adaptation of the cells to 
      fluid flow.
FAU - Kreke, Michelle R
AU  - Kreke MR
AD  - Department of Chemical Engineering, Virginia Polytechnic Institute and State 
      University, Blacksburg, VA 24061-0211, USA.
FAU - Huckle, William R
AU  - Huckle WR
FAU - Goldstein, Aaron S
AU  - Goldstein AS
LA  - eng
GR  - R03-DE15915-01/DE/NIDCR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Bone
JT  - Bone
JID - 8504048
RN  - 0 (Glycerophosphates)
RN  - 0 (Ibsp protein, rat)
RN  - 0 (Integrin-Binding Sialoprotein)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sialoglycoproteins)
RN  - 0 (Spp1 protein, rat)
RN  - 106441-73-0 (Osteopontin)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 3.1.1.34 (Lipoprotein Lipase)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - K7Q1JQR04M (Dinoprostone)
RN  - PQ6CK8PD0R (Ascorbic Acid)
RN  - WWH06G87W6 (beta-glycerophosphoric acid)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Ascorbic Acid/pharmacology
MH  - Bone Marrow Cells/cytology/metabolism/*physiology
MH  - Cell Count
MH  - Cells, Cultured
MH  - Dexamethasone/pharmacology
MH  - Dinoprostone/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Glycerophosphates/pharmacology
MH  - Integrin-Binding Sialoprotein
MH  - Lipoprotein Lipase/genetics
MH  - Microfluidics
MH  - Osteopontin
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sialoglycoproteins/*genetics/metabolism
MH  - Stromal Cells/cytology/metabolism/*physiology
MH  - Time Factors
EDAT- 2005/05/05 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/05/05 09:00
PHST- 2004/08/27 00:00 [received]
PHST- 2005/03/01 00:00 [revised]
PHST- 2005/03/11 00:00 [accepted]
PHST- 2005/05/05 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/05/05 09:00 [entrez]
AID - S8756-3282(05)00099-2 [pii]
AID - 10.1016/j.bone.2005.03.008 [doi]
PST - ppublish
SO  - Bone. 2005 Jun;36(6):1047-55. doi: 10.1016/j.bone.2005.03.008.