PMID- 15870367
OWN - NLM
STAT- MEDLINE
DCOM- 20050615
LR  - 20240322
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 71
IP  - 5
DP  - 2005 May
TI  - Enumeration of respiring Pseudomonas spp. in milk within 6 hours by fluorescence 
      in situ hybridization following formazan reduction.
PG  - 2748-52
AB  - Respiring Pseudomonas spp. in milk were quantified within 6 h by fluorescence in 
      situ hybridization (FISH) with vital staining. FISH with an oligonucleotide probe 
      based on 16S rRNA sequences was used for the specific detection of Pseudomonas 
      spp. at the single cell level. 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) was 
      used to estimate bacterial respiratory activity. The numbers of respiring 
      Pseudomonas cells as determined by FISH with CTC staining (CTC-FISH) were almost 
      the same or higher than the numbers of CFU as determined by the conventional 
      culture method.
FAU - Kitaguchi, Akiko
AU  - Kitaguchi A
AD  - Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 
      565-0871, Japan.
FAU - Yamaguchi, Nobuyasu
AU  - Yamaguchi N
FAU - Nasu, Masao
AU  - Nasu M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Formazans)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - *Colony Count, Microbial
MH  - Formazans/*metabolism
MH  - *In Situ Hybridization, Fluorescence
MH  - Milk/*microbiology
MH  - Oxygen Consumption
MH  - Pseudomonas/growth & development/*isolation & purification
PMC - PMC1087560
EDAT- 2005/05/05 09:00
MHDA- 2005/06/16 09:00
PMCR- 2005/05/01
CRDT- 2005/05/05 09:00
PHST- 2005/05/05 09:00 [pubmed]
PHST- 2005/06/16 09:00 [medline]
PHST- 2005/05/05 09:00 [entrez]
PHST- 2005/05/01 00:00 [pmc-release]
AID - 71/5/2748 [pii]
AID - 1902-04 [pii]
AID - 10.1128/AEM.71.5.2748-2752.2005 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2005 May;71(5):2748-52. doi: 
      10.1128/AEM.71.5.2748-2752.2005.