PMID- 15871444
OWN - NLM
STAT- MEDLINE
DCOM- 20050526
LR  - 20111117
IS  - 0277-0903 (Print)
IS  - 0277-0903 (Linking)
VI  - 42
IP  - 2
DP  - 2005 Mar
TI  - Vaccination with DNA encoding human T-cell epitopes suppresses Der p induced 
      allergic responses in mice.
PG  - 119-25
AB  - The apparent complexity of allergen-specific T-cell response in terms of epitope 
      usage in humans is a potential barrier to peptide-based immunotherapy for 
      allergy. A knowledge of cross-reacting T-cell epitopes of common allergens might 
      have an impact on the development of vaccines for immunotherapy. We examined the 
      efficiency of vaccinating with plasmid DNA coding only human T-cell epitopes on 
      the suppression of allergic reactions in mice. BALB/c mice that received an 
      injection of mixed naked DNA plasmids encoding the five classes of human T-cell 
      epitopes on Der p 1 and Der p 2 produced a significant reduction in total and Der 
      p-specific immunoglobulin E (IgE) synthesis. In Der p specific-IgG2a antibody 
      responses, vaccinated mice showed more prominent responses than controls. Higher 
      levels of interferon-gamma, a Th1 cytokine associated with the suppression of IgE 
      production, were found in the sera of vaccinated mice. Histologic studies showed 
      a marked reduction in the infiltration of inflammatory cells in the lung tissues 
      of vaccinated mice vs. controls. These results suggest that vaccination with DNA 
      encoding human T-cell epitopes effectively inhibits allergic responses in mice 
      and might induce cross-regulation on helper T-cell level in vivo.
FAU - Kwon, Soon Seog
AU  - Kwon SS
AD  - Department of Internal Medicine, Catholic University of Korea, Medical College, 
      Seoul, Republic of Korea.
FAU - Kim, Nack Sung
AU  - Kim NS
FAU - Yoo, Tai June
AU  - Yoo TJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Asthma
JT  - The Journal of asthma : official journal of the Association for the Care of 
      Asthma
JID - 8106454
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Arthropod Proteins)
RN  - 0 (Dermatophagoides pteronyssinus antigen p 2)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.- (Dermatophagoides pteronyssinus antigen p 1)
SB  - IM
MH  - Animals
MH  - Antigens, Dermatophagoides/*immunology
MH  - Arthropod Proteins
MH  - Cysteine Endopeptidases
MH  - Desensitization, Immunologic/*methods
MH  - Epitopes, T-Lymphocyte/*genetics
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/biosynthesis
MH  - Immunoglobulin G/biosynthesis
MH  - Lung/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Plasmids
MH  - Treatment Outcome
EDAT- 2005/05/06 09:00
MHDA- 2005/05/27 09:00
CRDT- 2005/05/06 09:00
PHST- 2005/05/06 09:00 [pubmed]
PHST- 2005/05/27 09:00 [medline]
PHST- 2005/05/06 09:00 [entrez]
PST - ppublish
SO  - J Asthma. 2005 Mar;42(2):119-25.