PMID- 15871684
OWN - NLM
STAT- MEDLINE
DCOM- 20050823
LR  - 20081121
IS  - 1043-0342 (Print)
IS  - 1043-0342 (Linking)
VI  - 16
IP  - 4
DP  - 2005 Apr
TI  - Lentiviral vector-transduced dendritic cells induce specific T cell response in a 
      nonhuman primate model.
PG  - 527-32
AB  - Dendritic cells (DCs) are effective in stimulating and controlling the outcome of 
      T cell responses. Human immunodeficiency virus type 1-based lentiviral vectors 
      can achieve sustained transduction of genes/antigens in dividing and nondividing 
      cells, thus representing a candidate vector for stable expression of antigens in 
      DCs. We previously established conditions for transduction of purified cytokine 
      mobilized rhesus CD34(+) cells in vitro, and transplantation of the autologous 
      transduced cells in a nonhuman primate model in vivo. In the present study, we 
      transplanted DCs derived from EGFP-transduced CD34(+) cells into nonmyeloablated 
      rhesus macaques. Transplantation of DCs stably expressing EGFP into autologous 
      animals induces persistent, long-lived (up to 100 weeks) EGFP-specific T cell 
      responses. Of note, no humoral responses against EGFP are detected in the 
      transplanted animals. These studies provide, to our knowledge, the first 
      demonstration that lentiviral transduction of CD34(+) progenitor cells 
      subsequently differentiated to DCs is capable of priming a specific T cell 
      response in a nonhuman primate in vivo. Taken together, our data provide formal 
      in vivo evidence that lentivirus-transduced dendritic cells represent a potential 
      approach in eliciting cellular immune responses in primates.
FAU - Kung, Sam K P
AU  - Kung SK
AD  - Department of Microbiology and Immunology and Medicine, David Geffen School of 
      Medicine, UCLA, Los Angeles, CA 90095, USA.
FAU - Bonifacino, Aylin
AU  - Bonifacino A
FAU - Metzger, Mark E
AU  - Metzger ME
FAU - Ringpis, Gene-Errol
AU  - Ringpis GE
FAU - Donahue, Robert E
AU  - Donahue RE
FAU - Chen, Irvin S Y
AU  - Chen IS
LA  - eng
GR  - P30 AI28697/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Hum Gene Ther
JT  - Human gene therapy
JID - 9008950
RN  - 0 (Antigens, CD34)
RN  - 0 (Immune Sera)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibody Formation
MH  - Antigens, CD34/genetics/immunology
MH  - Cell Transplantation/methods
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/physiology
MH  - Genetic Vectors/adverse effects/genetics
MH  - Green Fluorescent Proteins/genetics/immunology
MH  - Humans
MH  - Immune Sera
MH  - Interferon-gamma/pharmacology
MH  - Lentivirus/*genetics
MH  - Macaca mulatta
MH  - Primates
MH  - Stem Cells/immunology/physiology
MH  - T-Lymphocytes/*immunology
MH  - Transduction, Genetic/*methods
EDAT- 2005/05/06 09:00
MHDA- 2005/08/24 09:00
CRDT- 2005/05/06 09:00
PHST- 2005/05/06 09:00 [pubmed]
PHST- 2005/08/24 09:00 [medline]
PHST- 2005/05/06 09:00 [entrez]
AID - 10.1089/hum.2005.16.527 [doi]
PST - ppublish
SO  - Hum Gene Ther. 2005 Apr;16(4):527-32. doi: 10.1089/hum.2005.16.527.