PMID- 15871725
OWN - NLM
STAT- MEDLINE
DCOM- 20050822
LR  - 20171116
IS  - 1320-5463 (Print)
IS  - 1320-5463 (Linking)
VI  - 55
IP  - 5
DP  - 2005 May
TI  - Distinguishing between proliferating nodal lymphoid blasts in chronic myelogenous 
      leukemia and non-Hodgkin lymphoma: report of three cases and detection of a 
      bcr/abl fusion signal by single-cell analysis.
PG  - 273-9
AB  - Lymph node biopsies were analyzed from three patients with chronic myelogenous 
      leukemia (CML) showing nodal blast proliferation. Immunohistochemically, the 
      blasts from all three patients had an immature marker profile with a T-blast 
      population (cCD3+, CD4-, CD7+, CD8-, CD99+, terminal deoxynucleotidyl transferase 
      +) and a hematopoietic progenitor cell marker (CD34). In two patients, the blasts 
      also expressed myeloid lineage specificity (naphthol AS-D chloroacetate esterase 
      activity and myeloperoxidase positivity). However, it was difficult to 
      distinguish between blast proliferation in CML and non-Hodgkin lymphoma from 
      these immunohistopathological findings alone. Subsequently, bcr gene 
      rearrangement and bcr/abl mRNA expression were detected by Southern blot and 
      reverse transcription-polymerase chain reaction analysis of the lymph nodes. 
      Fluorescence in situ hybridization (FISH) analysis of lymph node touch smears 
      also disclosed bcr/abl gene fusion signals in the blasts of all patients, 
      confirming that the blasts were derived from Philadelphia chromosome-positive 
      CML. Accurate discrimination between the proliferating nodal blasts of CML and 
      non-Hodgkin lymphoma is essential for determining subsequent therapy. FISH 
      analysis of bcr/abl in single-cell blast preparations is an efficient tool that 
      allows rapid, accurate cytopathological diagnosis of extramedullary blast-phase 
      CML and its discrimination from non-Hodgkin lymphoma.
FAU - Yashima-Abo, Akiko
AU  - Yashima-Abo A
AD  - Department of Pathology, School of Medicine, Iwate Medical University, Morioka, 
      Iwate, Japan. ayashima@iwate-med.ac.jp
FAU - Satoh, Takashi
AU  - Satoh T
FAU - Abo, Toru
AU  - Abo T
FAU - Aoki, Yusei
AU  - Aoki Y
FAU - Kowata, Shugo
AU  - Kowata S
FAU - Ito, Shigeki
AU  - Ito S
FAU - Ishida, Yoji
AU  - Ishida Y
FAU - Fujiwara, Hitomi
AU  - Fujiwara H
FAU - Maesawa, Chihaya
AU  - Maesawa C
FAU - Masuda, Tomoyuki
AU  - Masuda T
LA  - eng
PT  - Case Reports
PT  - Comparative Study
PT  - Journal Article
PL  - Australia
TA  - Pathol Int
JT  - Pathology international
JID - 9431380
RN  - 0 (12E7 Antigen)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD34)
RN  - 0 (Antigens, CD7)
RN  - 0 (CD3 Complex)
RN  - 0 (CD99 protein, human)
RN  - 0 (Cell Adhesion Molecules)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
RN  - EC 2.7.7.31 (DNA Nucleotidylexotransferase)
RN  - EC 3.1.- (Naphthol AS D Esterase)
SB  - IM
MH  - 12E7 Antigen
MH  - Adult
MH  - Antigens, CD/analysis
MH  - Antigens, CD34/analysis
MH  - Antigens, CD7/analysis
MH  - Blotting, Southern
MH  - CD3 Complex/analysis
MH  - Cell Adhesion Molecules/analysis
MH  - Cell Proliferation
MH  - DNA Nucleotidylexotransferase/analysis
MH  - Female
MH  - Fusion Proteins, bcr-abl/genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics/metabolism/*pathology
MH  - Lymph Nodes/chemistry/metabolism/*pathology
MH  - Lymphoma, Non-Hodgkin/genetics/metabolism/*pathology
MH  - Male
MH  - Middle Aged
MH  - Naphthol AS D Esterase/analysis
MH  - Peroxidase/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2005/05/06 09:00
MHDA- 2005/08/23 09:00
CRDT- 2005/05/06 09:00
PHST- 2005/05/06 09:00 [pubmed]
PHST- 2005/08/23 09:00 [medline]
PHST- 2005/05/06 09:00 [entrez]
AID - PIN1824 [pii]
AID - 10.1111/j.1440-1827.2005.01824.x [doi]
PST - ppublish
SO  - Pathol Int. 2005 May;55(5):273-9. doi: 10.1111/j.1440-1827.2005.01824.x.