PMID- 15875782
OWN - NLM
STAT- MEDLINE
DCOM- 20050908
LR  - 20061115
IS  - 0258-851X (Print)
IS  - 0258-851X (Linking)
VI  - 19
IP  - 3
DP  - 2005 May-Jun
TI  - Influence of hepatocyte growth factor/scatter factor (HGF/SF) on fibroblast 
      growth factor-2 (FGF-2) levels in external auditory canal cholesteatoma (EACC) 
      cell culture.
PG  - 599-603
AB  - BACKGROUND: In previous studies, we cited circulatory disorders and hypoxia as 
      etiological factors for the formation of external auditory canal cholesteatoma 
      (EACC) resulting in angiogenesis. Here, we investigate how the angiogenic factor 
      hepatocyte growth factor/scatter factor (HGF/SF) influences the level of another 
      angiogenic factor FGF-2. MATERIALS AND METHODS: After 16 to 72 hours of 
      incubation with 20 ng/ml HGF/SF, levels of VEGF in the HGF/SF-treated and 
      untreated culture was analyzed. We also investigated the influence of HGF/SF 
      (20-80 ng/ml) on the concentration of FGF-2. RESULTS: After 16 hours of 
      incubation with HGF/SF at 20 ng/ml, FGF-2 was measured at 44.19 pg/ml (control: 
      42.24 pg/ml). After 72 hours, FGF-2 was at 23.41 pg/ml (control: 14.83 pg/ml). 
      After 24 hours, 40 ng/ml HGF/SF showed the highest concentration of FGF-2. 
      CONCLUSION: FGF-2 levels were initially elevated after treatment with HGF/SF, 
      however, further incubation did not show any increase. We assume that HGF/SF 
      released FGF-2 in the matrix but did not induce FGF-2 expression. The most 
      effective HGF/SF concentration was 40 ng/ml.
FAU - Naim, Ramin
AU  - Naim R
AD  - Department of Otolaryngology, Head and Neck Surgery, University Hospital 
      Mannheim, D-68135 Mannheim, Germany. ramin.naim@hno.ma.uni-heidelberg.de
FAU - Chang, Ray C
AU  - Chang RC
FAU - Sadick, Haneen
AU  - Sadick H
FAU - Hormann, Karl
AU  - Hormann K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - In Vivo
JT  - In vivo (Athens, Greece)
JID - 8806809
RN  - 0 (Angiogenic Proteins)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Angiogenic Proteins/metabolism/pharmacology
MH  - Cells, Cultured
MH  - Cholesteatoma, Middle Ear/*physiopathology
MH  - Fibroblast Growth Factor 2/*metabolism
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Humans
MH  - Keratinocytes/drug effects/metabolism
MH  - Neovascularization, Pathologic
MH  - Skin/metabolism
MH  - Vascular Endothelial Growth Factor A/metabolism
EDAT- 2005/05/07 09:00
MHDA- 2005/09/09 09:00
CRDT- 2005/05/07 09:00
PHST- 2005/05/07 09:00 [pubmed]
PHST- 2005/09/09 09:00 [medline]
PHST- 2005/05/07 09:00 [entrez]
PST - ppublish
SO  - In Vivo. 2005 May-Jun;19(3):599-603.