PMID- 15875812
OWN - NLM
STAT- MEDLINE
DCOM- 20050725
LR  - 20190922
IS  - 1071-5762 (Print)
IS  - 1029-2470 (Linking)
VI  - 39
IP  - 1
DP  - 2005 Jan
TI  - Caloric restriction augments ROS defense in S. cerevisiae, by a Sir2p independent 
      mechanism.
PG  - 55-62
AB  - Aging is associated with increased production of reactive oxygen species (ROS) 
      and oxidation-induced damage to intracellular structures and membranes. Caloric 
      restriction (CR) has been demonstrated to delay aging in a variety of species. 
      Although the mechanisms of CR remain to be clearly elucidated, reductions in 
      oxidative damage have been shown to increase lifespan in several model systems. 
      Contrary to the general belief that ROS production is reduced in CR, this article 
      provides evidence that not only oxygen consumption but ROS production is enhanced 
      in the calorie restricted condition. To understand the biological mechanism 
      underlying the anti aging action of CR, the role of scavenging enzymes was 
      studied. It was found that super oxide dismutase (SOD1 and SOD2), catalase and 
      glutathione peroxidase (GPx) all are over expressed in CR. We further 
      investigated the role of Sir2, a potential effector of CR response in the 
      activation of scavenging enzymes. No marked difference was found in CR mediated 
      activation of SOD and catalase in the absence of Sir2. Our results suggest that 
      in CR scavenging enzymes are activated by a Sir2 independent manner.
FAU - Agarwal, Shruti
AU  - Agarwal S
AD  - Department of Biotechnology, National Institute of Pharmaceutical Education, and 
      Research Sector 67, S.A.S. Nagar, Punjab 160062, India.
FAU - Sharma, Siddharth
AU  - Sharma S
FAU - Agrawal, Vineet
AU  - Agrawal V
FAU - Roy, Nilanjan
AU  - Roy N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Free Radic Res
JT  - Free radical research
JID - 9423872
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Fluoresceins)
RN  - 0 (Free Radicals)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Oxidants)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Silent Information Regulator Proteins, Saccharomyces cerevisiae)
RN  - 2044-85-1 (diacetyldichlorofluorescein)
RN  - 298-83-9 (Nitroblue Tetrazolium)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 3.5.1.- (SIR2 protein, S cerevisiae)
RN  - EC 3.5.1.- (Sirtuin 2)
RN  - EC 3.5.1.- (Sirtuins)
RN  - EC 3.5.1.98 (Histone Deacetylases)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - *Caloric Restriction
MH  - Catalase
MH  - DNA, Ribosomal/metabolism
MH  - Energy Intake
MH  - Energy Metabolism
MH  - Fluoresceins/pharmacology
MH  - Free Radicals
MH  - Gene Silencing
MH  - Glutathione Peroxidase/metabolism
MH  - Histone Deacetylases/*physiology
MH  - Indicators and Reagents/pharmacology
MH  - Models, Biological
MH  - Models, Chemical
MH  - Nitroblue Tetrazolium/pharmacology
MH  - Oxidants/pharmacology
MH  - Oxidation-Reduction
MH  - Oxidative Stress
MH  - Oxygen/metabolism
MH  - Oxygen Consumption
MH  - *Reactive Oxygen Species
MH  - Saccharomyces cerevisiae/*metabolism
MH  - Silent Information Regulator Proteins, Saccharomyces cerevisiae/*physiology
MH  - Sirtuin 2
MH  - Sirtuins/*physiology
MH  - Superoxide Dismutase
MH  - Temperature
MH  - Time Factors
EDAT- 2005/05/07 09:00
MHDA- 2005/07/26 09:00
CRDT- 2005/05/07 09:00
PHST- 2005/05/07 09:00 [pubmed]
PHST- 2005/07/26 09:00 [medline]
PHST- 2005/05/07 09:00 [entrez]
AID - 10.1080/10715760400022343 [doi]
PST - ppublish
SO  - Free Radic Res. 2005 Jan;39(1):55-62. doi: 10.1080/10715760400022343.