PMID- 15876225
OWN - NLM
STAT- MEDLINE
DCOM- 20050802
LR  - 20061115
IS  - 0931-1793 (Print)
IS  - 0931-1793 (Linking)
VI  - 52
IP  - 3
DP  - 2005 Apr
TI  - Molecular typing of Mycobacterium bovis isolates from south-east Brazil by 
      spoligotyping and RFLP.
PG  - 129-33
AB  - The identification of 163 strains of Mycobacterium bovis by polymerase chain 
      reaction (PCR) and microbiological tests was carried out on 252 tuberculous-like 
      lesions (TLLs) collected from slaughtered cattle in south-east Brazil. This study 
      compared the usefulness of three genotyping techniques, IS6110-restriction 
      fragment length polymorphism (RFLP), polymorphic guanine-cytosine-rich sequence 
      (PGRS)-RFLP and direct repeat (DR)-spoligotyping, as applied to M. bovis 
      isolates. Based on IS6110-RFLP genotyping we selected a group of 23 isolates 
      containing more than one IS6110 copy, along with 16 samples containing one IS6110 
      copy from different geographical areas, evenly distributed among dairy (eight) 
      and beef cattle (eight). These selected isolates were analysed by PGRS-RFLP and 
      DR-spoligotyping genotyping. Dairy cattle (17%) display a higher frequency of 
      multiple IS6110 copies than beef cattle (10%). A comparison between the genotype 
      data obtained fails to show a correlation between the main clusters found by the 
      three techniques. However, the clustering of each genotyping procedure revealed 
      that the majority of strains are closely related. The RFLP-PGRS patterns showed a 
      sizable group (20.5%) containing a 5.5 kb fragment and the predominant 
      spoligotype is similar to that from the BCG vaccine strain. Unexpectedly, four 
      strains (2.4%) showed drug resistance to 0.2 microg/ml isoniazid and 20 microg/ml 
      ethionamide, but none of them was resistant to rifampicin or other antibiotics 
      tested.
FAU - Zanini, M S
AU  - Zanini MS
AD  - Center of Agrarian Science, Federal University of Espirito Santo, PO Box 16, 
      Alegre, ES, Brazil. zanini@cca.ufes.br
FAU - Moreira, E C
AU  - Moreira EC
FAU - Salas, C E
AU  - Salas CE
FAU - Lopes, M T P
AU  - Lopes MT
FAU - Barouni, A S
AU  - Barouni AS
FAU - Roxo, E
AU  - Roxo E
FAU - Telles, M A
AU  - Telles MA
FAU - Zumarraga, M J
AU  - Zumarraga MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Vet Med B Infect Dis Vet Public Health
JT  - Journal of veterinary medicine. B, Infectious diseases and veterinary public 
      health
JID - 100955260
SB  - IM
MH  - Animals
MH  - Bacterial Typing Techniques/*veterinary
MH  - Brazil
MH  - Cattle
MH  - Cluster Analysis
MH  - DNA Fingerprinting/veterinary
MH  - Drug Resistance, Bacterial/genetics
MH  - Mycobacterium bovis/*classification/*genetics
MH  - Phylogeny
MH  - *Polymorphism, Restriction Fragment Length
MH  - Tuberculosis, Bovine/*microbiology
EDAT- 2005/05/07 09:00
MHDA- 2005/08/03 09:00
CRDT- 2005/05/07 09:00
PHST- 2005/05/07 09:00 [pubmed]
PHST- 2005/08/03 09:00 [medline]
PHST- 2005/05/07 09:00 [entrez]
AID - JVB835 [pii]
AID - 10.1111/j.1439-0450.2005.00835.x [doi]
PST - ppublish
SO  - J Vet Med B Infect Dis Vet Public Health. 2005 Apr;52(3):129-33. doi: 
      10.1111/j.1439-0450.2005.00835.x.