PMID- 15877731
OWN - NLM
STAT- MEDLINE
DCOM- 20050614
LR  - 20071115
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 129
IP  - 4
DP  - 2005 May
TI  - Co-existence of multiple subclones in TEL-AML1 at diagnosis of acute 
      lymphoblastic leukaemia in association with submicroscopic deletion of AML1.
PG  - 491-8
AB  - The TEL/AML1 (ETV6/RUNX1) fusion gene is the most common genetic rearrangement in 
      paediatric acute lymphoblastic leukaemia (ALL). Although considered to be a 
      low-risk leukaemia, it is associated with a relapse rate of 10-20%. The 
      coexistence of different subclones at diagnosis, based on polymerase chain 
      reaction (PCR) studies of IG/TCR gene rearrangement, with differential response 
      to chemotherapy, was recently reported in this subtype of ALL. We wished to 
      demonstrate such subclones at diagnosis by a recently developed technique of 
      quantitative multiparametric fluorescence in situ hybridization (FISH). Bone 
      marrow cells from 80 paediatric patients with ALL at diagnosis were analysed for 
      the presence of the TEL/AML1 fusion gene by interphase FISH. Fourteen patients 
      were positive for the translocation. Four of them had several subclones 
      associated with various combinations of additional chromosomal abnormalities. The 
      most striking was an atypical and unexpected hybridization pattern consistent 
      with a submicroscopic deletion of the 5' region of the AML1 breakpoint. Other 
      abnormalities included TEL deletion, trisomy and tetrasomy 21 as well as double 
      TEL-AML1 fusion. The presence of numerous subclones in about 25% of patients with 
      TEL/AML1+ ALL suggests extensive clonal evolution by the time of diagnosis.
FAU - Rothman, Rachel
AU  - Rothman R
AD  - Department of Paediatric Haemato-Oncology and Institute of Haematology, The 
      Edmond and Lily Safra Children Hospital, The Chaim Sheba Medical Centre, 
      Tel-Hashomer, Israel.
FAU - Trakhtenbrot, Luba
AU  - Trakhtenbrot L
FAU - Bielorai, Bella
AU  - Bielorai B
FAU - Izraeli, Shai
AU  - Izraeli S
FAU - Ishoev, Galina
AU  - Ishoev G
FAU - Amariglio, Ninette
AU  - Amariglio N
FAU - Rechavi, Gideon
AU  - Rechavi G
FAU - Toren, Amos
AU  - Toren A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (Core Binding Factor Alpha 2 Subunit)
RN  - 0 (Genetic Markers)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (TEL-AML1 fusion protein)
SB  - IM
MH  - *Bone Marrow Cells
MH  - Child, Preschool
MH  - Cloning, Molecular
MH  - Core Binding Factor Alpha 2 Subunit
MH  - Female
MH  - Gene Deletion
MH  - Gene Rearrangement
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Infant
MH  - Interphase
MH  - Male
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
MH  - Prognosis
EDAT- 2005/05/10 09:00
MHDA- 2005/06/15 09:00
CRDT- 2005/05/10 09:00
PHST- 2005/05/10 09:00 [pubmed]
PHST- 2005/06/15 09:00 [medline]
PHST- 2005/05/10 09:00 [entrez]
AID - BJH5479 [pii]
AID - 10.1111/j.1365-2141.2005.05479.x [doi]
PST - ppublish
SO  - Br J Haematol. 2005 May;129(4):491-8. doi: 10.1111/j.1365-2141.2005.05479.x.