PMID- 15877963
OWN - NLM
STAT- MEDLINE
DCOM- 20091211
LR  - 20161124
IS  - 1003-0603 (Print)
IS  - 1003-0603 (Linking)
VI  - 17
IP  - 5
DP  - 2005 May
TI  - [Role of trefoil peptides in modulation of gastric adaptation to stress].
PG  - 302-6
AB  - OBJECTIVE: To investigate the role of trefoil peptides in modulation of gastric 
      adaptation to water restraint stress (WRS) in rats. METHODS: Wistar rats were 
      exposed to single or repeated WRS for 4 hours every other day for up to 6 days, 
      gastric mucosal blood flow (GMBF) was measured by LDF-3 flowmeter, the extent of 
      gastric mucosal lesions was evaluated grossly and histologically, and expression 
      of PS2 intestinal trefoil peptide (ITF), cyclooxygenase (COX-2), inducible nitric 
      oxide synthase (iNOS) and transferase growth factor-alpha (TGF-alpha) were 
      determined by reverse transcription-polymerase chain reaction (RT-PCR) and 
      Western blot. RESULTS: One application of WRS produced extensive gastric mucosal 
      erosion. With repeated WRS, the gastric mucosa became adapted to the stress of 
      WRS, and the ulcerative index (UI) was reduced by 22.0% that of one WRS challenge 
      after four consecutive WRS. Expression of PS2 was markedly decreased and 
      expression of ITF, COX-2, iNOS and TGF-alpha were markedly increased after single 
      stress. But this adaptation to WRS was accompanied by increased GMBF and active 
      cell proliferation in the neck region of gastric glands, and by increased 
      expression of PS2, ITF, TGF-alpha, but reduced expression of COX-2 and iNOS. 
      CONCLUSION: Gastric adaptation to WRS injury involves enhanced cell 
      proliferation, increased expression of PS2, ITF, TGF-alpha and reduced expression 
      of COX-2 and iNOS, suggesting trefoil peptides might play an important modulating 
      role in this phenomenon.
FAU - Nie, Shi-nan
AU  - Nie SN
AD  - Emergency Department, Nanjing General Hospital of Nanjing Command, Clinical 
      School of Medical College of Nanjing University, Nanjing 210002, Jiangsu, China.
FAU - Sun, Hai-chen
AU  - Sun HC
FAU - Wu, Xue-hao
AU  - Wu XH
FAU - Qian, Xiao-ming
AU  - Qian XM
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo 
      weizhongbing jijiuyixue
JID - 9887521
RN  - 0 (Neuropeptides)
RN  - 0 (Peptides)
RN  - 0 (RNA, Messenger)
RN  - 0 (TFF3 protein, rat)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 0 (Trefoil Factor-3)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, rat)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (Ptgs2 protein, rat)
SB  - IM
MH  - Animals
MH  - Cyclooxygenase 2/genetics/metabolism
MH  - Gastric Mucosa/blood supply/*metabolism/pathology
MH  - Neuropeptides/genetics/metabolism
MH  - Nitric Oxide Synthase Type II/genetics/metabolism
MH  - Peptides/metabolism/*physiology
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Regional Blood Flow
MH  - Stress, Physiological/*physiology
MH  - Transforming Growth Factor alpha/genetics/metabolism
MH  - Trefoil Factor-3
EDAT- 2005/05/10 09:00
MHDA- 2009/12/16 06:00
CRDT- 2005/05/10 09:00
PHST- 2005/05/10 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
PHST- 2005/05/10 09:00 [entrez]
PST - ppublish
SO  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2005 May;17(5):302-6.