PMID- 15880083
OWN - NLM
STAT- MEDLINE
DCOM- 20050706
LR  - 20190713
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 79
IP  - 9
DP  - 2005 May 15
TI  - Single-antigen-expressing cell lines are excellent tools for detecting human 
      leukocyte antigen-C-reactive antibodies in kidney transplant recipients.
PG  - 1268-72
AB  - BACKGROUND: Human leukocyte antigen (HLA)-C is expressed on nucleated cells and 
      platelets in lower levels than HLA-A,B, and its antigens are in linkage 
      disequilibrium with HLA-B antigens. Therefore, HLA-C antibody detection is 
      difficult. The authors questioned whether HLA-C could serve as a target in 
      clinical kidney transplantation using a newly developed assay. METHODS: Flow 
      cytometry was performed with sera from patients (n=34) awaiting a kidney 
      retransplant using nine cell lines expressing a single HLA-C antigen 
      (single-antigen lines [SAL]). RESULTS: The SAL were validated with HLA-C-specific 
      alloantisera and human monoclonal antibodies against HLA-A, -B, and -C. The 
      results were in agreement with the specificities previously reported. Exceptions, 
      because of new HLA-C specificities used here, could be explained by epitope 
      sharing between the antigens. With respect to patient sera, 15 of the 34 patients 
      tested (44%) showed serum reactivity toward one or more HLA-C SAL. CONCLUSIONS: 
      In contrast to peripheral blood lymphocytes, SAL are excellent targets for 
      detecting HLA-C-reactive alloantibodies by flow cytometry. This preliminary 
      analysis revealed that HLA-C-reactive antibodies are frequently present in sera 
      of retransplant patients, serving as possible targets in clinical 
      transplantation.
FAU - Zoet, Yvonne M
AU  - Zoet YM
AD  - Department of Immunohaematology and Blood Transfusion, Leiden University Medical 
      Center, Leiden, The Netherlands.
FAU - Eijsink, Chantal
AU  - Eijsink C
FAU - Bohmova, Radka
AU  - Bohmova R
FAU - Witvliet, Marian D
AU  - Witvliet MD
FAU - Kardol, Marrie J
AU  - Kardol MJ
FAU - Franke, Marry E I
AU  - Franke ME
FAU - Claas, Frans H J
AU  - Claas FH
FAU - Mulder, Arend
AU  - Mulder A
FAU - Doxiadis, Ilias I N
AU  - Doxiadis II
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Antibodies)
RN  - 0 (Antigens)
RN  - 0 (Biomarkers)
RN  - 0 (Epitopes)
RN  - 0 (HLA-C Antigens)
SB  - IM
MH  - Antibodies/blood
MH  - Antigens/*immunology
MH  - Biomarkers/analysis
MH  - Cell Line
MH  - Epitopes/analysis
MH  - HLA-C Antigens/*immunology
MH  - Humans
MH  - Kidney Transplantation/*immunology
MH  - Leukocytes/*immunology
MH  - Patient Selection
MH  - Reproducibility of Results
EDAT- 2005/05/10 09:00
MHDA- 2005/07/07 09:00
CRDT- 2005/05/10 09:00
PHST- 2005/05/10 09:00 [pubmed]
PHST- 2005/07/07 09:00 [medline]
PHST- 2005/05/10 09:00 [entrez]
AID - 00007890-200505150-00059 [pii]
AID - 10.1097/01.tp.0000161246.33019.b6 [doi]
PST - ppublish
SO  - Transplantation. 2005 May 15;79(9):1268-72. doi: 
      10.1097/01.tp.0000161246.33019.b6.