PMID- 15881372
OWN - NLM
STAT- MEDLINE
DCOM- 20100607
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 23
IP  - 1
DP  - 2005 Jan
TI  - [Measurement of monoamine transmitters in the brains of ovariectomized mice using 
      high performance liquid chromatography with fluorescence detection and gradient 
      flow rate elution].
PG  - 76-8
AB  - A method for the determination of norepinephrine (NE), dopamine (DA) and 
      5-hydroxytryptamine (5-HT) in brains of ovariectomized mice was established by 
      using high performance liquid chromatograph with a fluorescence detector, a C18 
      column and a gradient flow rate delivered by a single pump. The mobile phase was 
      a mixture of 0.1 mol/L KH2PO4 and CH3OH (9/1, v/v). Detection limits of the 
      method were determined to be lower than 10 nmol/L, absolute recoveries were above 
      80% and the relative standard deviations (RSDs) of intra-day were all within 
      3.03%. The method has the advantages of high sensitivity and of simple sample 
      preparation. Short analysis time and better separation of samples were also 
      obtained.
FAU - Zhang, Lei
AU  - Zhang L
AD  - Department of Biomedical Engineering of Zhejiang University, Hangzhou 310027, 
      China. zl78213@yahoo.com.cn
FAU - Lu, Yingjun
AU  - Lu Y
FAU - Deng, Tongle
AU  - Deng T
FAU - Zheng, Xiaoxiang
AU  - Zheng X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Biogenic Monoamines)
RN  - 333DO1RDJY (Serotonin)
RN  - VTD58H1Z2X (Dopamine)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Animals
MH  - Biogenic Monoamines/*analysis
MH  - Brain/*metabolism
MH  - Chemical Fractionation
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Dopamine/analysis
MH  - Female
MH  - *Fluorescence
MH  - Gravitation
MH  - Mice
MH  - Norepinephrine/analysis
MH  - Ovariectomy
MH  - Serotonin/analysis
EDAT- 2005/05/11 09:00
MHDA- 2010/06/09 06:00
CRDT- 2005/05/11 09:00
PHST- 2005/05/11 09:00 [pubmed]
PHST- 2010/06/09 06:00 [medline]
PHST- 2005/05/11 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2005 Jan;23(1):76-8.