PMID- 1588277
OWN - NLM
STAT- MEDLINE
DCOM- 19920623
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 175
IP  - 6
DP  - 1992 Jun 1
TI  - Major histocompatibility complex independent clonal T cell anergy by direct 
      interaction of Staphylococcus aureus enterotoxin B with the T cell antigen 
      receptor.
PG  - 1493-9
AB  - The Staphylococcal enterotoxin superantigens stimulate vigorous responses in T 
      cells bearing certain T cell antigen receptor (TCR) V beta regions. In addition 
      to activation, these superantigens also impart negative signals to T cells 
      resulting in a profound state of unresponsiveness or anergy. The Staphylococcus 
      aureus enterotoxins (SE) B and C2 bind to a closely related site on major 
      histocompatibility complex (MHC) human leukocyte antigen (HLA)-DR1 molecules. 
      Only SEB, however, interacts with the TCR V beta 3 region of HA1.7, a human 
      HLA-DR1 restricted T cell clone specific for influenza haemagglutinin. In 
      competition experiments, we demonstrated that the induction of anergy in HA1.7 by 
      SEB is unaffected by the presence of SEC2. These results suggest that SEB-induced 
      anergy is MHC independent and involves a direct interaction between the TCR and 
      SEB. To resolve definitively whether SEB binds directly to T cells in the absence 
      of MHC class II molecules, the cDNAs encoding the HA1.7 TCR were transfected into 
      an MHC class II-negative human T cell line. The addition of SEB to these 
      transfectants resulted in the downregulation of cell surface TCR expression, an 
      increase in the concentration of intracellular calcium ions, the production of 
      lymphokines, and reduced responsiveness to a subsequent challenge with SEB. We 
      conclude that SEB interacts directly with the TCR in the absence of cointeraction 
      with MHC class II molecules, and that this interaction may induce anergy in 
      HA1.7.
FAU - Hewitt, C R
AU  - Hewitt CR
AD  - Imperial Cancer Research Fund, London, UK.
FAU - Lamb, J R
AU  - Lamb JR
FAU - Hayball, J
AU  - Hayball J
FAU - Hill, M
AU  - Hill M
FAU - Owen, M J
AU  - Owen MJ
FAU - O'Hehir, R E
AU  - O'Hehir RE
LA  - eng
SI  - GENBANK/S40279
SI  - GENBANK/S44631
SI  - GENBANK/S44632
SI  - GENBANK/S44633
SI  - GENBANK/S44634
SI  - GENBANK/S44635
SI  - GENBANK/S44636
SI  - GENBANK/S44637
SI  - GENBANK/X63455
SI  - GENBANK/X63456
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antisense Elements (Genetics))
RN  - 0 (Enterotoxins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (Receptors, Antigen, T-Cell)
RN  - 39424-53-8 (enterotoxin B, staphylococcal)
RN  - SY7Q814VUP (Calcium)
RN  - VC2W18DGKR (Thymidine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Antisense Elements (Genetics)
MH  - Base Sequence
MH  - Binding Sites
MH  - Calcium/metabolism
MH  - Clone Cells
MH  - DNA Replication
MH  - Enterotoxins/*pharmacology
MH  - Humans
MH  - Kinetics
MH  - Macromolecular Substances
MH  - *Major Histocompatibility Complex
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Receptors, Antigen, T-Cell/drug effects/genetics/*immunology
MH  - Staphylococcus aureus/physiology
MH  - T-Lymphocytes/drug effects/*immunology
MH  - Thymidine
PMC - PMC2119262
EDAT- 1992/06/01 00:00
MHDA- 1992/06/01 00:01
PMCR- 1992/12/01
CRDT- 1992/06/01 00:00
PHST- 1992/06/01 00:00 [pubmed]
PHST- 1992/06/01 00:01 [medline]
PHST- 1992/06/01 00:00 [entrez]
PHST- 1992/12/01 00:00 [pmc-release]
AID - 92268797 [pii]
AID - 10.1084/jem.175.6.1493 [doi]
PST - ppublish
SO  - J Exp Med. 1992 Jun 1;175(6):1493-9. doi: 10.1084/jem.175.6.1493.