PMID- 15883041
OWN - NLM
STAT- MEDLINE
DCOM- 20050719
LR  - 20081121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 331
IP  - 4
DP  - 2005 Jun 17
TI  - Retroviral display of urokinase-binding domain fused to amphotropic envelope 
      protein.
PG  - 1485-93
AB  - Tumors frequently express urokinase (uPA) receptor (uPAR). To investigate whether 
      uPAR can efficiently target cancerous cells using amphotropic retroviral vectors, 
      we generated a retrovirus displaying the amino-terminal fragment (ATF) of uPA as 
      an N-terminal extension of viral envelope protein. We also made use of a 
      "two-step strategy" by inserting a uPA cleavage site between the ATF moiety and 
      the envelope. We measured the ability of ATF-bearing chimeric envelopes to infect 
      huPAR-overexpressing Madin-Darby canine kidney (MDCK) and control MDCK II cells. 
      The ATF-viruses infected both MDCK cell lines with an equivalent efficiency, 
      suggesting that the chimeric viruses were not sequestered by uPAR and infect 
      cells preferentially via the Pit-2 receptor. The addition of a uPA cleavage site 
      increased the infection level of huPAR-MDCK cells by 2-fold when uPA was present 
      in the infection medium. Surprisingly, ATF-env viruses infected huPAR-MDCK cells 
      5.5-fold more efficiently in the presence of exogenous uPA. This stimulatory 
      effect of uPA on infection of huPAR-MDCK cells by the ATF-env virus was 
      completely abolished by methyl-beta-cyclodextrin, suggesting that this effect 
      involves the caveolar endocytosis pathway.
FAU - Boucquey, Antoine
AU  - Boucquey A
AD  - EMI 00-20, INSERM and Universite Paris XI, Bat Gregory Pincus, 80 rue du General 
      Leclerc, 94276 Le Kremlin Bicetre Cedex, France.
FAU - Vilhardt, Frederik
AU  - Vilhardt F
FAU - Mitrovic, Tatjana
AU  - Mitrovic T
FAU - Franco, Dominique
AU  - Franco D
FAU - Weber, Anne
AU  - Weber A
FAU - Horellou, Philippe
AU  - Horellou P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA Primers)
RN  - 0 (Viral Envelope Proteins)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - DNA Primers
MH  - Dogs
MH  - Retroviridae/*genetics
MH  - Urokinase-Type Plasminogen Activator/*metabolism
MH  - Viral Envelope Proteins/*metabolism
EDAT- 2005/05/11 09:00
MHDA- 2005/07/20 09:00
CRDT- 2005/05/11 09:00
PHST- 2005/04/11 00:00 [received]
PHST- 2005/05/11 09:00 [pubmed]
PHST- 2005/07/20 09:00 [medline]
PHST- 2005/05/11 09:00 [entrez]
AID - S0006-291X(05)00858-2 [pii]
AID - 10.1016/j.bbrc.2005.04.075 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2005 Jun 17;331(4):1485-93. doi: 
      10.1016/j.bbrc.2005.04.075.