PMID- 15883814
OWN - NLM
STAT- MEDLINE
DCOM- 20050922
LR  - 20181113
IS  - 0171-5216 (Print)
IS  - 0171-5216 (Linking)
VI  - 131
IP  - 7
DP  - 2005 Jul
TI  - Macrophage level is not affected by monocyte chemotactic protein-1 in invasive 
      ductal breast carcinoma.
PG  - 453-8
AB  - PURPOSE AND METHOD: Monocyte chemotactic protein-1 (MCP-1) is a chemokine 
      involved in the macrophage infiltration of tumor tissue. Tumor-associated 
      macrophages (TAMs) are a population of mononuclear phagocytic cells that can have 
      a complex function in tumor biology. The aim of this study was to determine the 
      possible correlation between parenchymal MCP-1 expression and TAM level by 
      immunohistochemical analysis of 97 invasive ductal breast carcinomas, not 
      otherwise specified (NOS), and to investigate their relation with tumor size, 
      histological grade, mitotic activity index (MAI) and lymph node status. Secondly, 
      the MCP-1 mRNA was determined by reverse transcriptase-polymerase chain reaction 
      (RT-PCR) in eight samples of normal breast tissue and 27 samples of invasive 
      breast carcinomas and compared with TAMs. RESULTS: MCP-1 immunoreactivity was 
      present in tumor cells (17/97), but also in TAMs, fibroblasts and endothelial 
      cells. The statistical analysis did not show a significant correlation between 
      MCP-1 expression in tumoral epithelium and tumor size, histological grade, MAI, 
      lymph node status or TAMs. The results of RT-PCR showed that, in all cases of 
      breast carcinomas (27/27) and the majority of normal breast tissues (7/8), the 
      number of detected MCP-1 cDNA copies was above the detection limit. However, 
      carcinomas showed higher levels of MCP-1 mRNA than normal breast tissue. 
      Nevertheless, the statistical analysis did not find a significant correlation 
      between MCP-1 expression and macrophage infiltrations. CONCLUSION: These results 
      indicate that MCP-1 is probably not the only and/or crucial factor involved in 
      macrophage attraction to tumor locus in breast carcinoma.
FAU - Valkovic, T
AU  - Valkovic T
AD  - Department of Internal Medicine, Medical Faculty, University of Rijeka, Rijeka, 
      Croatia.
FAU - Fuckar, D
AU  - Fuckar D
FAU - Stifter, S
AU  - Stifter S
FAU - Matusan, K
AU  - Matusan K
FAU - Hasan, M
AU  - Hasan M
FAU - Dobrila, F
AU  - Dobrila F
FAU - Jonjic, N
AU  - Jonjic N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050510
PL  - Germany
TA  - J Cancer Res Clin Oncol
JT  - Journal of cancer research and clinical oncology
JID - 7902060
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Breast/metabolism/pathology
MH  - Breast Neoplasms/genetics/*metabolism/pathology
MH  - Carcinoma, Ductal, Breast/genetics/*metabolism/pathology
MH  - Chemokine CCL2/genetics/*metabolism
MH  - DNA/genetics/metabolism
MH  - Endothelium, Vascular/metabolism/pathology
MH  - Female
MH  - Fibroblasts/metabolism/pathology
MH  - Humans
MH  - Macrophages/*metabolism/pathology
MH  - Neoplasm Invasiveness/*pathology
MH  - Prognosis
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2005/05/11 09:00
MHDA- 2005/09/24 09:00
CRDT- 2005/05/11 09:00
PHST- 2004/07/06 00:00 [received]
PHST- 2004/12/12 00:00 [accepted]
PHST- 2005/05/11 09:00 [pubmed]
PHST- 2005/09/24 09:00 [medline]
PHST- 2005/05/11 09:00 [entrez]
AID - 10.1007/s00432-004-0667-3 [doi]
PST - ppublish
SO  - J Cancer Res Clin Oncol. 2005 Jul;131(7):453-8. doi: 10.1007/s00432-004-0667-3. 
      Epub 2005 May 10.