PMID- 15884116
OWN - NLM
STAT- MEDLINE
DCOM- 20050721
LR  - 20190430
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 11
IP  - 18
DP  - 2005 May 14
TI  - Blood platelet and monocyte activations and relation to stages of liver 
      cirrhosis.
PG  - 2754-8
AB  - AIM: Blood platelets (plt) and monocytes are the cells that play a crucial role 
      in the pathogenesis of liver damage and liver cirrhosis (LC). In this paper, the 
      analysis of mutual relationship between platelets and monocytes activation in LC 
      was conducted. METHODS: Immunofluorescent flow cytometry was used to measure the 
      percentage of activated platelet populations (CD62P, CD63), the percentage of 
      plt-monocyte aggregates (pma) (CD41/CD45), and activated monocytes (CD11b, CD14, 
      CD16) in the blood of 20 volunteers and 40 patients with LC. Platelet activation 
      markers: sP-selectin, platelet factor 4 (PF4), beta-thromboglobulin (betaTG) and 
      monocyte chemotactic peptide-1 (MCP-1) were measured and compared in different 
      stages of LC. RESULTS: Platelet activation with the increase in both betaTG serum 
      concentration and elevation of plt population (CD62P and CD63 as well as MIF 
      CD62P and CD63) is elevated as LC develops and thrombocytopenia rises. There is a 
      positive correlation between medial intensity of fluorescence (MIF) CD62P and MIF 
      CD63 in LC. We did not show any relationship between monocyte activation and pma 
      level. SP-selectin concentration correlates positively with plt count and pma, 
      and negatively with stage of plt activation and MIF CD62P and MIF CD63. There was 
      no correlation between MCP-1 concentration and plt, monocyte activation as well 
      as pma level in LC. CD16 monocytes and MIF CD16 populations are significantly 
      higher in the end stage of LC. A positive correlation occurs between the value of 
      CD11b monocyte population and MIF CD14 and MIF CD16 on monocytes in LC. 
      CONCLUSION: Platelet and monocyte activation plays an important role in LC. 
      Platelet activation stage does not influence monocyte activation and production 
      of plt aggregates with monocytes in LC. With LC development, thrombocytopenia may 
      be the result of plt consumption in platelet-monocyte aggregates.
FAU - Panasiuk, Anatol
AU  - Panasiuk A
AD  - Department of Infectious Diseases, Medical University of Bialystok, Zurawia Str. 
      14, 15-540 Bialystok, Poland. anatol@panasiuk.pl
FAU - Zak, Janusz
AU  - Zak J
FAU - Kasprzycka, Edwina
AU  - Kasprzycka E
FAU - Janicka, Katarzyna
AU  - Janicka K
FAU - Prokopowicz, Danuta
AU  - Prokopowicz D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
SB  - IM
MH  - Adult
MH  - Cell Aggregation
MH  - Female
MH  - Humans
MH  - Liver Cirrhosis/blood/complications/*physiopathology
MH  - Male
MH  - Middle Aged
MH  - *Monocytes
MH  - *Platelet Activation
MH  - Platelet Aggregation
MH  - Thrombocytopenia/etiology
PMC - PMC4305910
EDAT- 2005/05/11 09:00
MHDA- 2005/07/22 09:00
PMCR- 2005/05/14
CRDT- 2005/05/11 09:00
PHST- 2005/05/11 09:00 [pubmed]
PHST- 2005/07/22 09:00 [medline]
PHST- 2005/05/11 09:00 [entrez]
PHST- 2005/05/14 00:00 [pmc-release]
AID - 10.3748/wjg.v11.i18.2754 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2005 May 14;11(18):2754-8. doi: 10.3748/wjg.v11.i18.2754.