PMID- 15887216
OWN - NLM
STAT- MEDLINE
DCOM- 20051101
LR  - 20151119
IS  - 1043-3074 (Print)
IS  - 1043-3074 (Linking)
VI  - 27
IP  - 7
DP  - 2005 Jul
TI  - Expression of ets-1 transcription factor in human head and neck squamous cell 
      carcinoma and effect of histamine on metastatic potential of invasive tumor 
      through the regulation of expression of ets-1 and matrix metalloproteinase-3.
PG  - 585-96
AB  - BACKGROUND: Ets-1 controls the expression of critical genes involved in matrix 
      remodeling. The matrix metalloproteinase-3 (MMP-3) and urokinase type plasminogen 
      activator (uPA) are typical ets-1 responsive genes. Recent studies have shown an 
      increase in histamine synthesis and content in various human neoplasias. We 
      hypothesized that the increased local histamine overproduction contributed to 
      activation of matrix remodeling through the activation of MMP-3 expression of 
      peritumoral fibroblasts by means of ets-1 regulation in head and neck squamous 
      cell carcinomas (HNSCCs). METHODS: Paraffin-embedded sections of 30 HNSCCs were 
      immunostained for ets-1. The presence of ets-1 and MMP-3 mRNA in tumor samples 
      was confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR). To 
      simulate stromal reaction in vitro, cultured human mucosal fibroblast was used. 
      The level of ets-1 and MMP-3 mRNA was compared by use of RT-PCR, as was their 
      protein with flow-cytometry, in the presence or absence of basic fibroblast 
      growth factor (bFGF) (10 ng/mL) and histamine (1 microM). RESULTS: Correlation 
      between ets-1 expression and clinicopathologic background was not significant. In 
      all cases, expression of ets-1 was seen in the stroma. In in vitro study, 
      histamine upregulates production of ets-1 and MMP-3 in cultured fibroblast, and 
      bFGF can stimulate histamine expression in fibroblast. Immunofluorescence 
      staining supported the results of RT-PCR and flow cytometry. CONCLUSIONS: Ets-1 
      expression in HNSCCs has no prognostic value; however, ets-1 plays an important 
      role in tumor-host interaction. Histamine may accelerate the spread of HNSCC 
      through an ets-1-related mechanism.
FAU - Horvath, Barnabas
AU  - Horvath B
AD  - Department of Ear, Nose and Throat Diseases, National Medical Center 1135, 
      Szabolcs u. 35, Budapest, Hungary. bhorvath@ogyik.hu
FAU - Hegyesi, Hargita
AU  - Hegyesi H
FAU - Nagy, Pal
AU  - Nagy P
FAU - Falus, Andras
AU  - Falus A
FAU - Schaff, Zsuzsa
AU  - Schaff Z
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Head Neck
JT  - Head & neck
JID - 8902541
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (ETS1 protein, human)
RN  - 0 (Proto-Oncogene Protein c-ets-1)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-ets)
RN  - 0 (Transcription Factors)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 820484N8I3 (Histamine)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Carcinoma, Squamous Cell/genetics/*metabolism/secondary
MH  - Cell Culture Techniques
MH  - Female
MH  - Fibroblast Growth Factor 2/metabolism
MH  - Flow Cytometry
MH  - Head and Neck Neoplasms/genetics/*metabolism/pathology
MH  - Histamine/*pharmacology
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Matrix Metalloproteinase 3/genetics/*metabolism
MH  - Neoplasm Invasiveness
MH  - Proto-Oncogene Protein c-ets-1
MH  - Proto-Oncogene Proteins/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-ets
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription Factors/genetics/*metabolism
MH  - Up-Regulation/drug effects
EDAT- 2005/05/12 09:00
MHDA- 2005/11/03 09:00
CRDT- 2005/05/12 09:00
PHST- 2005/05/12 09:00 [pubmed]
PHST- 2005/11/03 09:00 [medline]
PHST- 2005/05/12 09:00 [entrez]
AID - 10.1002/hed.20188 [doi]
PST - ppublish
SO  - Head Neck. 2005 Jul;27(7):585-96. doi: 10.1002/hed.20188.