PMID- 15888066 OWN - NLM STAT- MEDLINE DCOM- 20050901 LR - 20230124 IS - 1600-6135 (Print) IS - 1600-6135 (Linking) VI - 5 IP - 6 DP - 2005 Jun TI - A tissue-engineered artificial bile duct grown to resemble the native bile duct. PG - 1541-7 AB - The aim of this study was to fabricate an artificial bile duct for the development of a new treatment for biliary diseases. Eighteen hybrid pigs were implanted with a bile duct organoid unit (BDOU) made of a bioabsorbable polymer. Twelve of the transplanted BDOUs had been seeded with autologous bone marrow cells (BMCs) in advance. Six animals, the controls, were grafted with the scaffold alone with no BMCs seeded. The common bile duct was cut, the hepatic cut end of the native common bile duct was anastomosed to the BDOU and the other end was anastomosed to the duodenum. The controls underwent a similar operation. The neo-bile duct was removed at pre-determined time points and investigated histologically. All 18 recipient pigs survived until their sacrifice at 6 weeks, 10 weeks or 6 months. Histological examination revealed incomplete epithelialization of the neo-bile duct at 6 weeks and 10 weeks after transplantation. At 6 months, the organoid exhibited a morphology almost identical to that of the native common bile duct. No differences were found between the controls and BMC-seeded pigs. These results show that the artificial bile duct thus fabricated can serve as a substitute for the native bile duct. FAU - Miyazawa, Mitsuo AU - Miyazawa M AD - Department of Surgery, Saitama Medical School, Japan. miyazawa@saitama-med.ac.jp FAU - Torii, Takahiro AU - Torii T FAU - Toshimitsu, Yasuko AU - Toshimitsu Y FAU - Okada, Katsuya AU - Okada K FAU - Koyama, Isamu AU - Koyama I FAU - Ikada, Yoshito AU - Ikada Y LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - Am J Transplant JT - American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons JID - 100968638 SB - IM MH - *Absorbable Implants MH - Animals MH - Bile Ducts/*surgery/*transplantation MH - *Stents MH - Swine MH - Time Factors MH - *Tissue Engineering MH - Transplantation, Autologous EDAT- 2005/05/13 09:00 MHDA- 2005/09/02 09:00 CRDT- 2005/05/13 09:00 PHST- 2005/05/13 09:00 [pubmed] PHST- 2005/09/02 09:00 [medline] PHST- 2005/05/13 09:00 [entrez] AID - S1600-6135(22)03702-9 [pii] AID - 10.1111/j.1600-6143.2005.00845.x [doi] PST - ppublish SO - Am J Transplant. 2005 Jun;5(6):1541-7. doi: 10.1111/j.1600-6143.2005.00845.x.