PMID- 15890245
OWN - NLM
STAT- MEDLINE
DCOM- 20050712
LR  - 20101118
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 223
IP  - 1
DP  - 2005 Jun 1
TI  - Gastric cancer cell detection in peritoneal lavage: RT-PCR for carcinoembryonic 
      antigen transcripts versus the combined cytology with peritoneal carcinoembryonic 
      antigen levels.
PG  - 129-35
AB  - Free cancer cells exfoliated from the serosal surfaces of primary cancers are 
      considered to be responsible for peritoneal dissemination, which are both the 
      most frequent pattern of treatment failure and the most important cause of death 
      in gastric cancer patients. Detection of free cancer cells in the peritoneal 
      cavity at the time of surgery, therefore, is thought to be of great value in 
      predicting peritoneal recurrence and accordingly the prognosis of gastric cancer 
      patients. This study was designed to determine whether free cancer cells in 
      peritoneal lavage fluid from gastric cancer patients could be identified by a 
      reverse-transcriptase polymerase chain reaction (RT-PCR) method specific to 
      carcinoembryonic antigen (CEA) mRNA. Simultaneously, the results from 
      conventional cytological examination were evaluated and the levels of CEA in 
      peritoneal lavage were determined. Of the 40 gastric cancer patients enrolling in 
      this investigation, 11 (27.5%) were positive for CEA mRNA in their peritoneal 
      lavage, whereas only 6 (15%) and 8 (20%) were shown to be positive by cytological 
      examination and peritoneal CEA (pCEA) assay, respectively. Furthermore, RT-PCR 
      positive for CEA mRNA was correlated with the depth of tumor invasion (P<0.001), 
      lymph node metastases (P=0.004), the TNM stage (P<0.001) and peritoneal 
      recurrence (P<0.001). The technique of RT-PCR was more sensitive than 
      conventional cytological examination and pCEA levels in the detection of 
      peritoneal free cancer cells as well as in the prediction of peritoneal 
      recurrence. In addition, CEA RT-PCR had a high concordance rate (82.5%) with the 
      combination of cytology with pCEA levels. These observations suggest that it is 
      feasible to identify free cancer cells in peritoneal lavage by using a CEA 
      mRNA-specific RT-PCR method, and this assay can be a promising diagnostic 
      modality for evaluating the risk of peritoneal dissemination in gastric cancer 
      patients following operations.
FAU - Wang, Jaw-Yuan
AU  - Wang JY
AD  - Department of Surgery, Kaohsiung Medical University and Hospital, 100 Tzyou 1st 
      Road, Kaohsiung 807, Taiwan, ROC.
FAU - Lin, Shiu-Ru
AU  - Lin SR
FAU - Lu, Chien-Yu
AU  - Lu CY
FAU - Chen, Chung-Chi
AU  - Chen CC
FAU - Wu, Deng-Chyang
AU  - Wu DC
FAU - Chai, Chee-Yin
AU  - Chai CY
FAU - Chen, Fang-Ming
AU  - Chen FM
FAU - Hsieh, Jan-Sing
AU  - Hsieh JS
FAU - Huang, Tsung-Jen
AU  - Huang TJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041028
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (Carcinoembryonic Antigen)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Carcinoembryonic Antigen/analysis/*genetics
MH  - Female
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Peritoneal Cavity/*pathology
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
MH  - Stomach Neoplasms/diagnosis/*pathology
MH  - Therapeutic Irrigation
EDAT- 2005/05/14 09:00
MHDA- 2005/07/13 09:00
CRDT- 2005/05/14 09:00
PHST- 2004/07/26 00:00 [received]
PHST- 2004/09/16 00:00 [revised]
PHST- 2004/09/20 00:00 [accepted]
PHST- 2005/05/14 09:00 [pubmed]
PHST- 2005/07/13 09:00 [medline]
PHST- 2005/05/14 09:00 [entrez]
AID - S0304-3835(04)00739-6 [pii]
AID - 10.1016/j.canlet.2004.09.031 [doi]
PST - ppublish
SO  - Cancer Lett. 2005 Jun 1;223(1):129-35. doi: 10.1016/j.canlet.2004.09.031. Epub 
      2004 Oct 28.