PMID- 15892700
OWN - NLM
STAT- MEDLINE
DCOM- 20050809
LR  - 20061115
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 7
IP  - 6
DP  - 2005 Jun
TI  - Physiological and molecular characterization of a microbial community established 
      in unsaturated, petroleum-contaminated soil.
PG  - 806-18
AB  - The microbial communities established in soil samples from an unsaturated, 
      petroleum-contaminated zone and from an adjacent uncontaminated site were 
      characterized by physiological and molecular approaches. Possible electron 
      acceptors such as sulfate and nitrate had been completely depleted in these soil 
      samples. Slurries of these soil samples were incubated in bottles in the presence 
      of hydrocarbon indicators (benzene, toluene, xylene and decane), and the 
      degradation of these compounds was examined. Supplementation with electron 
      acceptors stimulated hydrocarbon degradation, although the stimulatory effect was 
      small in the contaminated soil. The initial degradation rates in the contaminated 
      soil under fermentative/methanogenic conditions were comparable to those under 
      aerobic conditions. The microbial populations in the original soil samples were 
      analysed by cloning and sequencing of polymerase chain reaction (PCR)-amplified 
      bacterial and archaeal 16S rRNA gene fragments, showing that the sequences 
      retrieved from these soils were substantially different. For instance, 
      Epsilonproteobacteria, Gammaproteobacteria, Crenarchaeota and Methanosarcinales 
      could only be detected at significant levels in the contaminated soil. Denaturing 
      gradient gel electrophoresis (DGGE) analyses of 16S rRNA gene fragments amplified 
      by PCR from the incubated soil-slurry samples showed that supplementation of the 
      electron acceptors resulted in a shift in the major populations, while the DGGE 
      profiles after incubating the contaminated soil under the 
      fermentative/methanogenic conditions were not substantially changed. These 
      results suggest that petroleum contamination of the unsaturated zone resulted in 
      the establishment of a fermentative/methanogenic community with substantial 
      hydrocarbon-degrading potential.
FAU - Kasai, Yuki
AU  - Kasai Y
AD  - Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan. 
      yuki.kasai@mbio.jp
FAU - Takahata, Yoh
AU  - Takahata Y
FAU - Hoaki, Toshihiro
AU  - Hoaki T
FAU - Watanabe, Kazuya
AU  - Watanabe K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Petroleum)
RN  - 0 (Soil Pollutants)
SB  - IM
MH  - Bacteria/classification/*drug effects/growth & development
MH  - Biodegradation, Environmental
MH  - DNA, Bacterial/analysis/*drug effects/genetics
MH  - DNA, Ribosomal/genetics
MH  - *Ecosystem
MH  - Electrophoresis, Agar Gel/methods
MH  - Environmental Exposure/adverse effects
MH  - Petroleum/*toxicity
MH  - *Soil Microbiology
MH  - Soil Pollutants/*toxicity
EDAT- 2005/05/17 09:00
MHDA- 2005/08/10 09:00
CRDT- 2005/05/17 09:00
PHST- 2005/05/17 09:00 [pubmed]
PHST- 2005/08/10 09:00 [medline]
PHST- 2005/05/17 09:00 [entrez]
AID - EMI754 [pii]
AID - 10.1111/j.1462-2920.2005.00754.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2005 Jun;7(6):806-18. doi: 10.1111/j.1462-2920.2005.00754.x.