PMID- 15892718
OWN - NLM
STAT- MEDLINE
DCOM- 20050928
LR  - 20171116
IS  - 0893-5785 (Print)
IS  - 0893-5785 (Linking)
VI  - 18
IP  - 3
DP  - 2005 Jun
TI  - An oxygen transporter hemocyanin can act on the late pathway of melanin 
      synthesis.
PG  - 214-9
AB  - 5,6-Dihydroxyindole (DHI) and 5,6-dihydroxyindole-2-carboxylic acid (DHICA) are 
      precursors of eumelanin. The effects of crustacean hemolymph proteins on these 
      eumelanin-related metabolites were investigated. Zymogram analysis indicated that 
      polymers of hemocyanin (Hc) subunits converted DHI into black pigment while no 
      effects were observed using DHICA as a substrate. Spectrum changes for mixtures 
      of purified Hc and DHI showed a profile similar to oxidized DHI by mushroom 
      tyrosinase while Hc had only slight effects on DHICA. Typical inhibitors of 
      tyrosinase and phenoloxidase severely hampered the production of oxidized DHI. 
      Taken together with previous results, these data indicate that Hc plays a crucial 
      role in the conversion of DHI in the hemolymph of crustaceans, which promotes 
      late reactions in the melanin synthetic pathway as well as early reactions 
      (oxidation of tyrosine and DOPA to dopaquinone).
FAU - Adachi, Kohsuke
AU  - Adachi K
AD  - Division of Applied Biosciences, Graduate School of Agriculture, Kyoto 
      University, Japan.
FAU - Wakamatsu, Kazumasa
AU  - Wakamatsu K
FAU - Ito, Shosuke
AU  - Ito S
FAU - Miyamoto, Nari
AU  - Miyamoto N
FAU - Kokubo, Takeshi
AU  - Kokubo T
FAU - Nishioka, Takaaki
AU  - Nishioka T
FAU - Hirata, Takashi
AU  - Hirata T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Pigment Cell Res
JT  - Pigment cell research
JID - 8800247
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Indoles)
RN  - 0 (Melanins)
RN  - 4790-08-3 (5,6-dihydroxy-2-indolylcarboxylic acid)
RN  - 9013-72-3 (Hemocyanins)
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
RN  - S88TT14065 (Oxygen)
RN  - Z3OC8499KG (5,6-dihydroxyindole)
SB  - IM
MH  - Animals
MH  - Enzyme Inhibitors/pharmacology
MH  - Hemocyanins/chemistry/*metabolism
MH  - Hemolymph/metabolism
MH  - Hydrogen-Ion Concentration
MH  - Indoles/antagonists & inhibitors/chemistry/metabolism
MH  - Melanins/*biosynthesis/metabolism
MH  - Monophenol Monooxygenase/antagonists & inhibitors
MH  - Oxygen/*metabolism
MH  - Penaeidae
MH  - Structure-Activity Relationship
EDAT- 2005/05/17 09:00
MHDA- 2005/09/29 09:00
CRDT- 2005/05/17 09:00
PHST- 2005/05/17 09:00 [pubmed]
PHST- 2005/09/29 09:00 [medline]
PHST- 2005/05/17 09:00 [entrez]
AID - PCR232 [pii]
AID - 10.1111/j.1600-0749.2005.00232.x [doi]
PST - ppublish
SO  - Pigment Cell Res. 2005 Jun;18(3):214-9. doi: 10.1111/j.1600-0749.2005.00232.x.