PMID- 15893591
OWN - NLM
STAT- MEDLINE
DCOM- 20050802
LR  - 20181201
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 136
IP  - 1-2
DP  - 2005 May 20
TI  - TRPM8 protein localization in trigeminal ganglion and taste papillae.
PG  - 91-8
AB  - TRPM8 is a TRP family cation channel which can be activated by cold stimuli or 
      l-menthol. However, TRPM8 protein localization of nerve terminals in sensory 
      organs remains unknown. Here we generated an antibody against TRPM8 and analyzed 
      TRPM8 protein localization in trigeminal ganglia (TG) and in sensory nerve fibers 
      in the tongue. TRPM8 immunoreactivity was detected in a subset of neurons with a 
      small diameter in TG and in nerve fibers in the tongue. TRPM8-immunoreactive 
      nerve fibers were rich in fungiform papillae, but sparse in foliate and 
      circumvallate papillae. The TRPM8-immunoreactive nerve fibers reached the outer 
      epithelial layer in each papilla, while no TRPM8-immunoreactive nerve fibers 
      penetrated into taste buds. Double labeling analysis revealed that TRPM8 
      immunoreactivity was co-expressed with a part of TRPV1 or CGRP-immunoreactive 
      neurons in TG. However, TRPM8 immunoreactivity was not observed in TRPV1- or 
      CGRP-positive nerve fibers in fungiform, foliate, and circumvallate papillae. 
      These results suggest that TRPM8 protein is present in sensory lingual nerve 
      fibers mainly projected from TG and might work as cold and l-menthol receptors on 
      tongue.
FAU - Abe, Junji
AU  - Abe J
AD  - Division of Biological Information, Department of Intelligence Science and 
      Technology, Graduate School of Informatics, Kyoto University, Yoshida-honmachi, 
      Sakyo-ku, Kyoto 606-8501, Japan.
FAU - Hosokawa, Hiroshi
AU  - Hosokawa H
FAU - Okazawa, Makoto
AU  - Okazawa M
FAU - Kandachi, Mikako
AU  - Kandachi M
FAU - Sawada, Yosuke
AU  - Sawada Y
FAU - Yamanaka, Keiji
AU  - Yamanaka K
FAU - Matsumura, Kiyoshi
AU  - Matsumura K
FAU - Kobayashi, Shigeo
AU  - Kobayashi S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050302
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Ion Channels)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Neurofilament Proteins)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (TRPM Cation Channels)
RN  - 0 (TRPM8 protein, human)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (Trpm8 protein, rat)
RN  - 0 (Trpv1 protein, rat)
RN  - 108688-71-7 (neurofilament protein H)
RN  - EC 3.4.19.12 (UCHL1 protein, rat)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Blotting, Western/methods
MH  - Calcitonin Gene-Related Peptide/metabolism
MH  - Cell Line
MH  - Gene Expression Regulation/*physiology
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - Ion Channels/*metabolism
MH  - Male
MH  - Neoplasm Proteins/*metabolism
MH  - Neurofilament Proteins/metabolism
MH  - Neurons/metabolism
MH  - Proto-Oncogene Proteins c-myc/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - TRPM Cation Channels
MH  - TRPV Cation Channels
MH  - Taste Buds/*metabolism
MH  - Tongue/*cytology/metabolism
MH  - Transfection/methods
MH  - Trigeminal Ganglion/cytology/*metabolism
MH  - Ubiquitin Thiolesterase/metabolism
EDAT- 2005/05/17 09:00
MHDA- 2005/08/03 09:00
CRDT- 2005/05/17 09:00
PHST- 2004/08/24 00:00 [received]
PHST- 2004/12/30 00:00 [revised]
PHST- 2005/01/09 00:00 [accepted]
PHST- 2005/05/17 09:00 [pubmed]
PHST- 2005/08/03 09:00 [medline]
PHST- 2005/05/17 09:00 [entrez]
AID - S0169-328X(05)00034-3 [pii]
AID - 10.1016/j.molbrainres.2005.01.013 [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 2005 May 20;136(1-2):91-8. doi: 
      10.1016/j.molbrainres.2005.01.013. Epub 2005 Mar 2.