PMID- 15893840
OWN - NLM
STAT- MEDLINE
DCOM- 20051121
LR  - 20141120
IS  - 0168-3659 (Print)
IS  - 0168-3659 (Linking)
VI  - 105
IP  - 1-2
DP  - 2005 Jun 20
TI  - Heparin-loaded zein microsphere film and hemocompatibility.
PG  - 120-31
AB  - Zein was studied as a drug-eluting coating film composed of zein microspheres for 
      cardiovascular devices (e.g. stent). In vitro 
      3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) analysis 
      showed that both zein film and its degraded product had better biocompatibility 
      compared with Corning culture plate on the growth of human umbilical veins 
      endothelial cells (HUVECs, p<0.05, n=6), and the effect of zein degraded product 
      on HUVECs was dose-dependent. The best result was obtained at 0.3 mg/ml of the 
      addition. The encapsulation efficiency of heparin and heparin loading varied with 
      the amount of both zein and heparin, and the highest encapsulation efficiency 
      (heparin 1.33 mg/ml and zein 16 mg/ml) was 22.77+/-1.33% (n=3). Scanning electron 
      microscope (SEM) observation indicated that the zein film was made of 
      microspheres in diameter from nano- to micrometer, which could be controlled. 
      Sizes of heparin-loaded zein microspheres changed before and after release of 
      heparin because of conglutination among zein microspheres. Release rate of 
      heparin from microsphere film reached to 33.5+/-1.2% within 12 h, and began to 
      get into subsequent "slow release" phase; about 55% of the entrapped heparin was 
      released after 20 days. Both zein film and heparin-loaded zein microsphere film 
      were effective in suppressing platelet adhesion, and the heparin-loaded film 
      showed a better anticoagulation as determined with thrombin time (TT) assay. 
      These results suggest that zein film could be used directly as a new type of 
      coating material for its better biocompatibility with HUVECs. Moreover, the 
      heparin-loaded zein microsphere film can significantly improve the 
      hemocompatibility.
FAU - Wang, Hua-Jie
AU  - Wang HJ
AD  - School of Life Science and Biotechnology, Shanghai Jiaotong University, 1954 
      Huashan Road, Shanghai 200030, China.
FAU - Lin, Zhi-Xin
AU  - Lin ZX
FAU - Liu, Xin-Ming
AU  - Liu XM
FAU - Sheng, Shi-Yan
AU  - Sheng SY
FAU - Wang, Jin-Ye
AU  - Wang JY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Control Release
JT  - Journal of controlled release : official journal of the Controlled Release 
      Society
JID - 8607908
RN  - 0 (Anticoagulants)
RN  - 0 (Blood Proteins)
RN  - 0 (Excipients)
RN  - 9005-49-6 (Heparin)
RN  - 9010-66-6 (Zein)
SB  - IM
MH  - Anticoagulants/*administration & dosage
MH  - Blood Platelets/chemistry
MH  - Blood Proteins/chemistry
MH  - Cell Adhesion
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Endothelial Cells/metabolism
MH  - Excipients
MH  - Heparin/*administration & dosage
MH  - Humans
MH  - In Vitro Techniques
MH  - Microscopy, Electron, Scanning
MH  - Microspheres
MH  - Particle Size
MH  - Zein/*chemistry
EDAT- 2005/05/17 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/05/17 09:00
PHST- 2004/06/05 00:00 [received]
PHST- 2005/03/21 00:00 [revised]
PHST- 2005/03/25 00:00 [accepted]
PHST- 2005/05/17 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/05/17 09:00 [entrez]
AID - S0168-3659(05)00136-7 [pii]
AID - 10.1016/j.jconrel.2005.03.014 [doi]
PST - ppublish
SO  - J Control Release. 2005 Jun 20;105(1-2):120-31. doi: 
      10.1016/j.jconrel.2005.03.014.