PMID- 15893973
OWN - NLM
STAT- MEDLINE
DCOM- 20060816
LR  - 20061115
IS  - 0012-1606 (Print)
IS  - 0012-1606 (Linking)
VI  - 281
IP  - 2
DP  - 2005 May 15
TI  - Dynamic rearrangement of telomeres during spermatogenesis in mice.
PG  - 196-207
AB  - Chromosomal structure within the nucleus influences various biological processes 
      such as transcription and replication. Telomeres are located at the end of 
      eukaryotic chromosomes and they can be a decisive factor for correct chromosomal 
      positioning. To gain new insight into telomere dynamics, we examined telomere 
      length and positional changes during spermatogenesis using improved fluorescence 
      in situ hybridization (FISH) and in situ telomeric repeat amplification protocols 
      (TRAP) on histological sections. FISH revealed telomere length and chromosome 
      position within nuclei change dynamically. Telomere extension occurred during 
      spermiogenesis. In situ TRAP analysis verified elevated telomerase activity in 
      elongating spermatids. Together, these data show that elongated spermatids have 
      longer telomeres than precursor spermatogenic cells. This observation indicates 
      that telomere elongation in haploid cells occurs after meiosis and in the absence 
      of genomic replication. Analyses of testes from telomerase null mice further 
      support the significance of telomere dynamics during spermatogenesis and the 
      existence of an alternative telomere extension pathway.
FAU - Tanemura, Kentaro
AU  - Tanemura K
AD  - Brain Development Research Group, Brain Science Institute, Institute of Physical 
      and Chemical Research (RIKEN), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
FAU - Ogura, Atsuo
AU  - Ogura A
FAU - Cheong, Cheolho
AU  - Cheong C
FAU - Gotoh, Hideo
AU  - Gotoh H
FAU - Matsumoto, Kazuya
AU  - Matsumoto K
FAU - Sato, Eimei
AU  - Sato E
FAU - Hayashi, Yoshihiro
AU  - Hayashi Y
FAU - Lee, Han-Woong
AU  - Lee HW
FAU - Kondo, Takashi
AU  - Kondo T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dev Biol
JT  - Developmental biology
JID - 0372762
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Animals
MH  - Cell Nucleus/physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Spermatogenesis/*physiology
MH  - Telomerase/genetics/*metabolism
MH  - Telomere/*physiology
MH  - Testis/metabolism/ultrastructure
EDAT- 2005/05/17 09:00
MHDA- 2006/08/17 09:00
CRDT- 2005/05/17 09:00
PHST- 2004/10/08 00:00 [received]
PHST- 2005/02/10 00:00 [revised]
PHST- 2005/02/23 00:00 [accepted]
PHST- 2005/05/17 09:00 [pubmed]
PHST- 2006/08/17 09:00 [medline]
PHST- 2005/05/17 09:00 [entrez]
AID - S0012-1606(05)00131-4 [pii]
AID - 10.1016/j.ydbio.2005.02.025 [doi]
PST - ppublish
SO  - Dev Biol. 2005 May 15;281(2):196-207. doi: 10.1016/j.ydbio.2005.02.025.