PMID- 15894296
OWN - NLM
STAT- MEDLINE
DCOM- 20050715
LR  - 20131121
IS  - 0006-2952 (Print)
IS  - 0006-2952 (Linking)
VI  - 70
IP  - 1
DP  - 2005 Jul 1
TI  - Functional expression of particular isoforms of excitatory amino acid 
      transporters by rodent cartilage.
PG  - 70-81
AB  - In the present study, we have attempted to demonstrate functional expression by 
      the rodent cartilage of particular isoforms of excitatory amino acid transporters 
      (EAATs) essentially required for central glutamatergic signal termination. 
      Constitutive expression of mRNA was shown for the first time with the neuronal 
      EAAT subtype excitatory amino acid carrier-1 (EAAC1), in addition to glial 
      subtypes such as glutamate aspartate transporter (GLAST) and glutamate 
      transporter-1 (GLT-1), in rat costal chondrocytes cultured for 7-21 days on 
      reverse transcription polymerase chain reaction (RT-PCR). Western blotting 
      analysis confirmed the expression of corresponding proteins for both GLAST and 
      GLT-1 in cultured chondrocytes. The accumulation of [(3)H]glutamate (Glu) 
      occurred in a temperature- and sodium-dependent manner with biochemical and 
      pharmacological profiles similar to those seen for brain EAATs in chondrocytes 
      cultured for 7 days, while [(3)H]Glu accumulation consisted of a single component 
      with a K(m) of 39.1+/-2.3 microM and a V(max) of 1320+/-120pmol/mg protein/min, 
      respectively. In organotypic cultured metatarsals isolated before vascularization 
      from embryonic mice, where cells underwent maturational development from resting 
      to proliferating, prehypertrophic, hypertrophic and calcified chondrocytes in a 
      progressive order of cellular differentiation, moreover, mRNA expression was seen 
      for GLAST, GLT-1 and EAAT4 but not for EAAC1 subtypes. Immunohistochemical 
      analysis revealed distribution profiles different from each other with GLAST, 
      GLT-1 and EAAT4 isoforms in sections of cultured metatarsals and isolated tibiae. 
      These results suggest that extracellular Glu could be cleared up into 
      intracellular locations through particular glial and/or neuronal EAAT isoforms 
      functionally expressed by the rodent cartilage.
FAU - Hinoi, Eiichi
AU  - Hinoi E
AD  - Laboratory of Molecular Pharmacology, Division of Pharmaceutical Sciences, 
      Kanazawa University Graduate School of Natural Science and Technology, Kanazawa, 
      Ishikawa 920-1192, Japan.
FAU - Wang, Liyang
AU  - Wang L
FAU - Takemori, Akihiro
AU  - Takemori A
FAU - Yoneda, Yukio
AU  - Yoneda Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem Pharmacol
JT  - Biochemical pharmacology
JID - 0101032
RN  - 0 (Amino Acid Transport System X-AG)
RN  - 0 (Carrier Proteins)
RN  - 0 (Excitatory Amino Acid Transporter 1)
RN  - 0 (Excitatory Amino Acid Transporter 2)
RN  - 0 (Excitatory Amino Acid Transporter 3)
RN  - 0 (Excitatory Amino Acid Transporter 4)
RN  - 0 (Glutamate Plasma Membrane Transport Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Slc1a1 protein, mouse)
RN  - 0 (Slc1a1 protein, rat)
RN  - 0 (Slc1a3 protein, mouse)
RN  - 0 (Slc1a3 protein, rat)
RN  - 0 (Slc1a6 protein, mouse)
RN  - 0 (Slc1a6 protein, rat)
RN  - 0 (Symporters)
RN  - 3KX376GY7L (Glutamic Acid)
SB  - IM
MH  - Amino Acid Transport System X-AG/genetics
MH  - Animals
MH  - Carrier Proteins/*genetics
MH  - Cells, Cultured
MH  - Chondrocytes/*metabolism
MH  - Excitatory Amino Acid Transporter 1/genetics
MH  - Excitatory Amino Acid Transporter 2/genetics
MH  - Excitatory Amino Acid Transporter 3
MH  - Excitatory Amino Acid Transporter 4
MH  - Female
MH  - Glutamate Plasma Membrane Transport Proteins
MH  - Glutamic Acid/*metabolism
MH  - Mice
MH  - Protein Isoforms
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Wistar
MH  - Symporters/genetics
EDAT- 2005/05/17 09:00
MHDA- 2005/07/16 09:00
CRDT- 2005/05/17 09:00
PHST- 2005/03/07 00:00 [received]
PHST- 2005/04/04 00:00 [revised]
PHST- 2005/04/08 00:00 [accepted]
PHST- 2005/05/17 09:00 [pubmed]
PHST- 2005/07/16 09:00 [medline]
PHST- 2005/05/17 09:00 [entrez]
AID - S0006-2952(05)00225-X [pii]
AID - 10.1016/j.bcp.2005.04.025 [doi]
PST - ppublish
SO  - Biochem Pharmacol. 2005 Jul 1;70(1):70-81. doi: 10.1016/j.bcp.2005.04.025.