PMID- 15894450
OWN - NLM
STAT- MEDLINE
DCOM- 20051115
LR  - 20131121
IS  - 0731-7085 (Print)
IS  - 0731-7085 (Linking)
VI  - 39
IP  - 1-2
DP  - 2005 Sep 1
TI  - Separation and simultaneous determination of rutin, puerarin, daidzein, esculin 
      and esculetin in medicinal preparations by non-aqueous capillary.
PG  - 125-31
AB  - A simple method for the simultaneous determination of five bioactive components 
      (rutin, puerarin, daidzein esculin and esculetin) in traditional medicinal 
      preparations by non-aqueous capillary electrophoresis with UV detection has been 
      developed for the first time. A running buffer composed of 15% acetonitrile, 2.5% 
      acetic acid and 90 mM sodium cholate in methanol was found to be the most 
      suitable for this separation. The limits of detection for five analytes were over 
      the range of 0.050-1.216 microg ml(-1). The relative standard deviations 
      (R.S.Ds.) of the migration times and the peak areas of the analytes were in the 
      range of 1.3-2.9% and 2.2-2.7% (intraday), 1.7-1.9% and 2.8-3.6% (interday), 
      respectively. In the tested concentration range, linear relationships 
      (correlation coefficients: 0.9974 for rutin, 0.9976 for puerarin, 0.9981 for 
      daidzein, 0.9972 for esculin and 0.9929 for esculetin) between peak areas and 
      concentrations of the analytes were obtained. This method has been successfully 
      applied to simultaneous determination of the five bioactive components with 
      recoveries over the range of 89.4-107.4%.
FAU - Li, Cunhong
AU  - Li C
AD  - Department of Chemistry, Lanzhou University, PR China.
FAU - Chen, Anjia
AU  - Chen A
FAU - Chen, Xiaofeng
AU  - Chen X
FAU - Chen, Xingguo
AU  - Chen X
FAU - Hu, Zhide
AU  - Hu Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pharm Biomed Anal
JT  - Journal of pharmaceutical and biomedical analysis
JID - 8309336
RN  - 0 (Isoflavones)
RN  - 0 (Umbelliferones)
RN  - 1Y1L18LQAF (Esculin)
RN  - 5G06TVY3R7 (Rutin)
RN  - 6287WC5J2L (daidzein)
RN  - SM2XD6V944 (esculetin)
RN  - Z9W8997416 (puerarin)
SB  - IM
MH  - Electrophoresis, Capillary/*methods
MH  - Esculin/*analysis
MH  - Isoflavones/*analysis
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Rutin/*analysis
MH  - Sensitivity and Specificity
MH  - Spectrophotometry, Ultraviolet/*methods
MH  - Umbelliferones/*analysis
EDAT- 2005/05/17 09:00
MHDA- 2005/11/16 09:00
CRDT- 2005/05/17 09:00
PHST- 2004/11/22 00:00 [received]
PHST- 2005/03/30 00:00 [revised]
PHST- 2005/03/30 00:00 [accepted]
PHST- 2005/05/17 09:00 [pubmed]
PHST- 2005/11/16 09:00 [medline]
PHST- 2005/05/17 09:00 [entrez]
AID - S0731-7085(05)00227-X [pii]
AID - 10.1016/j.jpba.2005.03.018 [doi]
PST - ppublish
SO  - J Pharm Biomed Anal. 2005 Sep 1;39(1-2):125-31. doi: 10.1016/j.jpba.2005.03.018.