PMID- 15894585
OWN - NLM
STAT- MEDLINE
DCOM- 20051006
LR  - 20220321
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 78
IP  - 2
DP  - 2005 Aug
TI  - Polysaccharide purified from Ganoderma lucidum induced activation and maturation 
      of human monocyte-derived dendritic cells by the NF-kappaB and p38 
      mitogen-activated protein kinase pathways.
PG  - 533-43
AB  - Ganoderma lucidum, a fungus native to China, has been widely used to promote 
      health and longevity in the Chinese. The polysaccharide component with a branched 
      (1-->6)-beta-D-glucan moiety of G. lucidum (PS-G) has been reported to exert 
      anti-tumor activity and activation of natural killer cells. In this study, we 
      investigated the effects of PS-G on human monocyte-derived dendritic cells (DC). 
      Treatment of DC with PS-G resulted in the enhanced cell-surface expression of 
      CD80, CD86, CD83, CD40, CD54, and human leukocyte antigen (HLA)-DR, as well as 
      the enhanced production of interleukin (IL)-12p70, p40, and IL-10 and also 
      IL-12p35, p40, and IL-10 mRNA expression, and the capacity for endocytosis was 
      suppressed in DC. In addition, treatment of DC with PS-G resulted in enhanced T 
      cell-stimulatory capacity and increased T cell secretion of interferon-gamma and 
      IL-10. Neutralization with antibodies against Toll-like receptor (TLR)-4 
      inhibited the PS-G-induced production of IL-12 p40 and IL-10, suggesting a vital 
      role for TLR-4 in signaling DC upon incubation with PS-G. Further study showed 
      that PS-G was able to augment inhibitor of kappaB (IkappaB) kinase and nuclear 
      factor (NF)-kappaB activity and also IkappaB alpha and p38 mitogen-activated 
      protein kinase (MAPK) phosphorylation. Further, inhibition of NF-kappaB by 
      helenalin and p38 MAPK by SB98059 prevented the effects of PS-G in the expression 
      of CD80, CD86, CD83, CD40, CD54, and HLA-DR and production of IL-12p70, p40, and 
      IL-10 in various degrees. Taken together, our data demonstrate that PS-G can 
      effectively promote the activation and maturation of immature DC, suggesting that 
      PS-G may possess a potential in regulating immune responses.
FAU - Lin, Yu-Li
AU  - Lin YL
AD  - Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan 
      University, Taipei, Taiwan, ROC.
FAU - Liang, Yu-Chih
AU  - Liang YC
FAU - Lee, Shiuh-Sheng
AU  - Lee SS
FAU - Chiang, Bor-Luen
AU  - Chiang BL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050513
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, CD)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Interleukins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (NF-kappa B)
RN  - 0 (NFKBIA protein, human)
RN  - 0 (Polysaccharides)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptors)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Antibodies, Monoclonal/pharmacology
MH  - Antigens, CD/biosynthesis
MH  - Cell Differentiation/*drug effects/physiology
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*physiology
MH  - HLA-DR Antigens/biosynthesis
MH  - Humans
MH  - I-kappa B Proteins/metabolism
MH  - Interleukins/biosynthesis
MH  - MAP Kinase Signaling System/*drug effects/physiology
MH  - Membrane Glycoproteins/antagonists & inhibitors
MH  - Monocytes/cytology/physiology
MH  - NF-KappaB Inhibitor alpha
MH  - NF-kappa B/metabolism
MH  - Polysaccharides/chemistry/*isolation & purification/*pharmacology
MH  - Receptors, Cell Surface/antagonists & inhibitors
MH  - Reishi/*chemistry
MH  - Toll-Like Receptor 4
MH  - Toll-Like Receptors
MH  - p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors/metabolism
EDAT- 2005/05/17 09:00
MHDA- 2005/10/07 09:00
CRDT- 2005/05/17 09:00
PHST- 2005/05/17 09:00 [pubmed]
PHST- 2005/10/07 09:00 [medline]
PHST- 2005/05/17 09:00 [entrez]
AID - jlb.0804481 [pii]
AID - 10.1189/jlb.0804481 [doi]
PST - ppublish
SO  - J Leukoc Biol. 2005 Aug;78(2):533-43. doi: 10.1189/jlb.0804481. Epub 2005 May 13.