PMID- 15894745
OWN - NLM
STAT- MEDLINE
DCOM- 20051017
LR  - 20181113
IS  - 0032-0889 (Print)
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Linking)
VI  - 138
IP  - 2
DP  - 2005 Jun
TI  - Arabidopsis thickvein mutation affects vein thickness and organ vascularization, 
      and resides in a provascular cell-specific spermine synthase involved in vein 
      definition and in polar auxin transport.
PG  - 767-77
AB  - Polar auxin transport has been implicated in the induction of vascular tissue and 
      in the definition of vein positions. Leaves treated with chemical inhibitors of 
      polar auxin transport exhibited vascular phenotypes that include increased vein 
      thickness and vascularization. We describe a recessive mutant, thickvein (tkv), 
      which develops thicker veins in leaves and in inflorescence stems. The increased 
      vein thickness is attributable to an increased number of vascular cells. Mutant 
      plants have smaller leaves and shorter inflorescence stems, and this reduction in 
      organ size and height is accompanied by an increase in organ vascularization, 
      which appears to be attributable to an increase in the recruitment of cells into 
      veins. Furthermore, although floral development is normal, auxin transport in the 
      inflorescence stem is significantly reduced in the mutant, suggesting that the 
      defect in auxin transport is responsible for the vascular phenotypes. In the 
      primary root, the veins appear morphologically normal, but root growth in the tkv 
      mutant is hypersensitive to exogenous cytokinin. The tkv mutation was found to 
      reside in the ACL5 gene, which encodes a spermine synthase and whose expression 
      is specific to provascular cells. We propose that ACL5/TKV is involved in vein 
      definition (defining the boundaries between veins and nonvein regions) and in 
      polar auxin transport, and that polyamines are involved in this process.
FAU - Clay, Nicole K
AU  - Clay NK
AD  - Department of Molecular, Cellular, and Developmental Biology, Yale University, 
      New Haven, Connecticut 06520-8104, USA.
FAU - Nelson, Timothy
AU  - Nelson T
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20050513
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Indoleacetic Acids)
RN  - EC 2.5.1.22 (Spermine Synthase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Arabidopsis/anatomy & histology/*genetics/*metabolism
MH  - Arabidopsis Proteins/*genetics/metabolism
MH  - Biological Transport/genetics
MH  - Flowers/metabolism
MH  - Gene Expression Regulation, Plant/physiology
MH  - Indoleacetic Acids/*metabolism
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Plant Leaves/*anatomy & histology/genetics
MH  - Plant Stems/anatomy & histology/metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Spermine Synthase/*genetics/metabolism
PMC - PMC1150395
EDAT- 2005/05/17 09:00
MHDA- 2005/10/18 09:00
PMCR- 2006/06/01
CRDT- 2005/05/17 09:00
PHST- 2005/05/17 09:00 [pubmed]
PHST- 2005/10/18 09:00 [medline]
PHST- 2005/05/17 09:00 [entrez]
PHST- 2006/06/01 00:00 [pmc-release]
AID - pp.104.055756 [pii]
AID - 055756 [pii]
AID - 10.1104/pp.104.055756 [doi]
PST - ppublish
SO  - Plant Physiol. 2005 Jun;138(2):767-77. doi: 10.1104/pp.104.055756. Epub 2005 May 
      13.