PMID- 15895897
OWN - NLM
STAT- MEDLINE
DCOM- 20050726
LR  - 20170112
IS  - 0392-856X (Print)
IS  - 0392-856X (Linking)
VI  - 23
IP  - 2
DP  - 2005 Mar-Apr
TI  - Monocyte chemoattractant protein-1 activates a regional Th1 immunoresponse in 
      nephritis of MRL/lpr mice.
PG  - 239-42
AB  - OBJECTIVE: Monocyte chemoattractant protein-1 (MCP-1) is upregulated and recruits 
      and activates inflammatory cells in nephritis of MRL lpr mice. It has been shown 
      that anti-MCP-1 gene therapy is specifically effective in nephritis, while it was 
      apparent that an imbalance towards Th1 predominance accelerates nephritis in 
      MRL/lpr mice. The aim of this study was to clarify whether blockade of the MCP-1 
      signal by anti-MCP-1 gene therapy influences the Th1/Th2 balance in MRL/lpr mice. 
      METHOD: An NH2-terminal deletion mutant of the MCP-1 gene (7ND) was injected into 
      the skeletal muscles of MRL/Ipr mice with advanced stage nephritis to suppress 
      MCP-1 and its receptor (CCR2) signaling pathway. We evaluated the local tissue 
      production of cytokines in splenocytes and microdissected infiltrating cells 
      within the glomeruli or interstitium. RESULT: Although the production of 
      cytokines in splenocytes was not influenced by anti-MCP-1 gene therapy, kidney 
      glomeruli IL-12 mRNA production and interstitium-infiltrating cell production of 
      IL-12 and IFN-gamma mRNA were significantly reduced. CONCLUSION: The blockade of 
      MCP-1 gene therapy does not influence helper T cell polarization, but acts 
      directly on the regional Th1 immunoreaction in MRL/lpr mice.
FAU - Shimizu, S
AU  - Shimizu S
AD  - Department of Medicine and Biosystemic Science, Graduate School of Medical 
      Sciences, Kyushu University, Fukuoka, Japan.
FAU - Nakashima, H
AU  - Nakashima H
FAU - Karube, K
AU  - Karube K
FAU - Ohshima, K
AU  - Ohshima K
FAU - Egashira, K
AU  - Egashira K
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Clin Exp Rheumatol
JT  - Clinical and experimental rheumatology
JID - 8308521
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/antagonists & inhibitors/*genetics/metabolism
MH  - Disease Models, Animal
MH  - Gene Expression Regulation
MH  - *Genetic Therapy/methods
MH  - Humans
MH  - Interferon-gamma/genetics/metabolism
MH  - Interleukin-12/genetics/metabolism
MH  - Kidney Glomerulus/metabolism/pathology
MH  - Lupus Nephritis/genetics/metabolism/pathology/*therapy
MH  - Mice
MH  - Mice, Inbred MRL lpr
MH  - RNA, Messenger/biosynthesis
MH  - Th1 Cells/immunology/*metabolism
MH  - Th2 Cells/immunology/metabolism
EDAT- 2005/05/18 09:00
MHDA- 2005/07/27 09:00
CRDT- 2005/05/18 09:00
PHST- 2005/05/18 09:00 [pubmed]
PHST- 2005/07/27 09:00 [medline]
PHST- 2005/05/18 09:00 [entrez]
PST - ppublish
SO  - Clin Exp Rheumatol. 2005 Mar-Apr;23(2):239-42.