PMID- 15896200 OWN - NLM STAT- MEDLINE DCOM- 20050923 LR - 20111117 IS - 0001-2815 (Print) IS - 0001-2815 (Linking) VI - 65 IP - 6 DP - 2005 Jun TI - Complete coding sequences and haplotypic associations of HLA-B*0707, -B*1524, -B*4405, -B*4802, -DRB1*0409, -DRB1*0411, -DRB1*1115, -DRB1*1305, and the novel allele -DRB1*0709. Group-specific amplification of cDNA from DRB1 alleles associated to DRB3 and DRB4. PG - 529-38 AB - We present here the characterization of the complete coding sequences, previously unavailable, of the human leukocyte antigen (HLA) alleles B*0707, B*1524, B*4405, B*4802, DRB1*0409, DRB1*0411, DRB1*1115, DRB1*1305, and that of a new allele, DRB1*0709. For the isolation of cDNA from the DRB1 gene, we designed a novel set of polymerase chain reaction (PCR) primers that makes it possible to amplify separately the groups of DRB1 alleles associated to each of the DRB3 and DRB4 loci. The primary structures, functional features, evolutionary relationships, haplotypic associations, and population distributions of each of the nine HLA-B and -DRB1 alleles reported here are reviewed. FAU - Vilches, C AU - Vilches C AD - Servicio de Inmunologia, Hospital Universitario Puerta de Hierro, 28035 Madrid, Spain. incvilches@inicia.es FAU - Sepulveda, S AU - Sepulveda S FAU - Balas, A AU - Balas A FAU - Solis, R AU - Solis R FAU - Aviles, M J AU - Aviles MJ FAU - Estefania, E AU - Estefania E FAU - Gomez-Lozano, N AU - Gomez-Lozano N FAU - Vicario, J L AU - Vicario JL FAU - dePablo, R AU - dePablo R LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Tissue Antigens JT - Tissue antigens JID - 0331072 RN - 0 (3' Untranslated Regions) RN - 0 (DNA Primers) RN - 0 (DNA, Complementary) RN - 0 (HLA-B Antigens) RN - 0 (HLA-DR Antigens) RN - 0 (HLA-DRB1 Chains) RN - 0 (HLA-DRB3 Chains) RN - 0 (HLA-DRB4 Chains) SB - IM MH - 3' Untranslated Regions MH - Alleles MH - Amino Acid Sequence MH - Base Sequence MH - DNA Primers/chemistry MH - DNA, Complementary/metabolism MH - Evolution, Molecular MH - HLA-B Antigens/*genetics MH - HLA-DR Antigens/*genetics MH - HLA-DRB1 Chains MH - HLA-DRB3 Chains MH - HLA-DRB4 Chains MH - Haplotypes MH - Humans MH - Molecular Sequence Data MH - Polymerase Chain Reaction MH - Polymorphism, Single-Stranded Conformational MH - Reverse Transcriptase Polymerase Chain Reaction MH - Sequence Analysis, DNA MH - Sequence Homology, Amino Acid EDAT- 2005/05/18 09:00 MHDA- 2005/09/24 09:00 CRDT- 2005/05/18 09:00 PHST- 2005/05/18 09:00 [pubmed] PHST- 2005/09/24 09:00 [medline] PHST- 2005/05/18 09:00 [entrez] AID - TAN407 [pii] AID - 10.1111/j.1399-0039.2005.00407.x [doi] PST - ppublish SO - Tissue Antigens. 2005 Jun;65(6):529-38. doi: 10.1111/j.1399-0039.2005.00407.x.