PMID- 15896550
OWN - NLM
STAT- MEDLINE
DCOM- 20050818
LR  - 20090410
IS  - 0887-2333 (Print)
IS  - 0887-2333 (Linking)
VI  - 19
IP  - 5
DP  - 2005 Aug
TI  - The effects of some Mannich bases on heat shock proteins HSC70 and GRP75, and 
      thioredoxin and glutaredoxin levels in Jurkat cells.
PG  - 573-80
AB  - Mannich bases interact with cellular thiols and inactivate thioredoxin reductase. 
      In this study, the effects of cytotoxic mono-Mannich bases 2, 3 and cyclic 
      Mannich base C1 on the expression of cytoprotective heat shock proteins (HSC70 
      and GRP75) and on levels of thioredoxin (TRX) and glutaredoxin (GRX) were 
      investigated in Jurkat cells. Cells were exposed to the compounds for 24 h in 
      cell culture medium with 1% FBS. C1 and 2 increased the levels of HSC70 (200% of 
      control) in all the concentrations tested, but 3 did not affect HSC70 levels. 
      Whereas 3 increased GRP75 expression (123-154%), 2 and C1 either did not affect 
      (95-87% for 2, and 88% for C1) or slightly decreased GRP75 expression (82% for 2 
      and 67% for C1). Mannich bases generally decreased GRX levels (68%, 63-77% and 
      33-71% for 2, 3 and C1, respectively), but 3 increased GRX levels at 1 microg/ml 
      (142%). Whereas 2 and 3 decreased TRX levels (30-79% and 37-44% of control, 
      respectively), C1 increased the expression of TRX (156-201%). Our results suggest 
      that decreases in GRX and TRX due to the alkylating effects of Mannich bases 
      might have prevented cell division and decreased survival in Jurkat cells, which 
      could not be prevented by increased heat shock protein expression.
FAU - Gul, Mustafa
AU  - Gul M
AD  - Department of Physiology, Faculty of Medicine, University of Kuopio, 70211 
      Kuopio, Finland.
FAU - Atalay, Mustafa
AU  - Atalay M
FAU - Gul, Halise Inci
AU  - Gul HI
FAU - Nakao, Chitose
AU  - Nakao C
FAU - Lappalainen, Jani
AU  - Lappalainen J
FAU - Hanninen, Osmo
AU  - Hanninen O
LA  - eng
PT  - Journal Article
DEP - 20050422
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (GLRX protein, human)
RN  - 0 (Glutaredoxins)
RN  - 0 (HSC70 Heat-Shock Proteins)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (HSPA8 protein, human)
RN  - 0 (Mannich Bases)
RN  - 0 (Membrane Proteins)
RN  - 0 (glucose-regulated proteins)
RN  - 52500-60-4 (Thioredoxins)
RN  - EC 1.- (Oxidoreductases)
SB  - IM
MH  - Glutaredoxins
MH  - HSC70 Heat-Shock Proteins
MH  - HSP70 Heat-Shock Proteins/*analysis
MH  - Humans
MH  - Jurkat Cells
MH  - Mannich Bases/*pharmacology
MH  - Membrane Proteins/*analysis
MH  - Oxidoreductases/*analysis
MH  - Thioredoxins/*analysis
EDAT- 2005/05/18 09:00
MHDA- 2005/08/19 09:00
CRDT- 2005/05/18 09:00
PHST- 2004/06/30 00:00 [received]
PHST- 2005/01/02 00:00 [revised]
PHST- 2005/03/04 00:00 [accepted]
PHST- 2005/05/18 09:00 [pubmed]
PHST- 2005/08/19 09:00 [medline]
PHST- 2005/05/18 09:00 [entrez]
AID - S0887-2333(05)00035-4 [pii]
AID - 10.1016/j.tiv.2005.03.004 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2005 Aug;19(5):573-80. doi: 10.1016/j.tiv.2005.03.004. Epub 
      2005 Apr 22.