PMID- 15896977
OWN - NLM
STAT- MEDLINE
DCOM- 20060414
LR  - 20220705
IS  - 1043-6618 (Print)
IS  - 1043-6618 (Linking)
VI  - 52
IP  - 3
DP  - 2005 Sep
TI  - Ginkgo biloba extract ameliorates ischemia reperfusion-induced renal injury in 
      rats.
PG  - 216-22
AB  - There is increasing evidence to suggest that reactive oxygen metabolites (ROMs) 
      play a role in the pathogenesis of ischemia/reperfusion injury (I/R) in the 
      kidney. This study was designed to determine the possible protective effect of 
      Ginkgo biloba extract (EGb) on renal ischemia/reperfusion (I/R) injury. Wistar 
      albino rats were unilaterally nephrectomized, and 15 days later they were 
      subjected to 45 min of renal pedicle occlusion followed by 6 h of reperfusion. 
      Ginkgo biloba extract (EGb) (50 mg kg(-1) day(-1)) or saline was administered 
      twice, 15 min prior to ischemia and immediately before the reperfusion period. At 
      the end of the treatment period, all rats were decapitated. Kidney samples were 
      taken for histological examination or determination of the renal malondialdehyde 
      (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity and collagen 
      content. Production of reactive oxidants was monitored by chemiluminescence (CL) 
      assay. Creatinine and urea concentrations in blood were measured for the 
      evaluation of renal function. Tumor necrosis factor-alpha (TNF-alpha) and lactate 
      dehydrogenase (LDH) were also assayed in serum samples. Ischemia/reperfusion 
      caused a significant decrease in GSH level, which was accompanied with 
      significant increases in MDA level, MPO activity and collagen content of kidney 
      tissues. Similarly, serum BUN and creatinine levels, as well as LDH and 
      TNF-alpha, were elevated in the I/R group as compared to control group. On the 
      other hand, EGb treatment reversed all these biochemical indices, as well as 
      histopathological alterations, which were induced by I/R. The findings imply that 
      ROMs play a causal role in I/R-induced renal injury and EGb exerts renoprotective 
      effects probably by the radical scavenging and antioxidant activities.
FAU - Sener, Goksel
AU  - Sener G
AD  - Department of Pharmacology, School of Pharmacy, Marmara University, Tibbiye Cad., 
      34668 Istanbul, Turkey. gsener@marmara.edu.tr
FAU - Sener, Emre
AU  - Sener E
FAU - Sehirli, Ozer
AU  - Sehirli O
FAU - Ogunc, Ayliz Velioglu
AU  - Ogunc AV
FAU - Cetinel, Sule
AU  - Cetinel S
FAU - Gedik, Nursal
AU  - Gedik N
FAU - Sakarcan, Abdullah
AU  - Sakarcan A
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Pharmacol Res
JT  - Pharmacological research
JID - 8907422
RN  - 0 (Acridines)
RN  - 0 (Plant Extracts)
RN  - 0 (Protective Agents)
RN  - 2315-97-1 (10,10'-dimethyl-9,9'-biacridinium)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 5EXP385Q4F (Luminol)
RN  - 9007-34-5 (Collagen)
RN  - AYI8EX34EU (Creatinine)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Acridines/metabolism
MH  - Animals
MH  - Blood Urea Nitrogen
MH  - Collagen/metabolism
MH  - Creatinine/blood
MH  - Ginkgo biloba/*chemistry
MH  - Glutathione/metabolism
MH  - Kidney/drug effects/metabolism/pathology
MH  - Kidney Diseases/metabolism/pathology/*prevention & control
MH  - L-Lactate Dehydrogenase/blood
MH  - Luminol/metabolism
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Oxidative Stress/drug effects
MH  - Peroxidase/metabolism
MH  - Plant Extracts/*pharmacology
MH  - Protective Agents/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/metabolism/pathology/*prevention & control
EDAT- 2005/05/18 09:00
MHDA- 2006/04/15 09:00
CRDT- 2005/05/18 09:00
PHST- 2005/02/09 00:00 [received]
PHST- 2005/03/23 00:00 [revised]
PHST- 2005/03/23 00:00 [accepted]
PHST- 2005/05/18 09:00 [pubmed]
PHST- 2006/04/15 09:00 [medline]
PHST- 2005/05/18 09:00 [entrez]
AID - S1043-6618(05)00080-0 [pii]
AID - 10.1016/j.phrs.2005.03.006 [doi]
PST - ppublish
SO  - Pharmacol Res. 2005 Sep;52(3):216-22. doi: 10.1016/j.phrs.2005.03.006.