PMID- 15897980
OWN - NLM
STAT- MEDLINE
DCOM- 20051207
LR  - 20100324
IS  - 1008-682X (Print)
IS  - 1008-682X (Linking)
VI  - 7
IP  - 2
DP  - 2005 Jun
TI  - Birth after intracytoplasmic sperm injection of ejaculated spermatozoa from a man 
      with mosaic Klinefelter's syndrome.
PG  - 217-20
AB  - AIM: To report a birth after intracytoplasmic sperm injection (ICSI) of 
      ejaculated spermatozoa from a man with mosaic Klinefelter's syndrome detected by 
      fluorescence in situ hybridization (FISH) analysis. METHODS: A 35-year-old man 
      with a normal appearance consulted our hospital because of sterility over a 
      5-year period. Chromosome analysis showed low-incidence mosaic Klinefelter's 
      syndrome. Using FISH, 96 % hyperploidy of the lymphocytes was found. We examined 
      the sex chromosome of the ejaculated spermatozoa. Using FISH, we examined 200 
      ejaculated spermatozoa and no hyperploidy was found. RESULTS: The 33-year-old 
      female partner of the male patient underwent an uncomplicated controlled ovarian 
      hyperstimulation sequence using a combined recombinant-follicle stimulating 
      hormone (rec-FSH) + human menopausal gonadotrophin (hMG) protocol, following late 
      luteal phase pituitary down regulation. This culminated in the retrieval of seven 
      oocytes, six of which were fertilized with ICSI. One ICSI attempt led to clinical 
      pregnancy with a healthy baby girl. CONCLUSION: We report a male patient with 
      low-incidence mosaic Klinefelter's syndrome whose ejaculated spermatozoa were 
      identified as being haploid by FISH before ICSI, leading to the successful 
      pregnancy of his wife and the birth of a healthy baby girl.
FAU - Akashi, Takuya
AU  - Akashi T
AD  - Department of Urology, Faculty of Medicine, Toyama Medical and Pharmaceutical 
      University, Sugitani 2630, Toyama 930-0194, Japan. akashitakuya@mail.goo.ne.jp
FAU - Fuse, Hideki
AU  - Fuse H
FAU - Kojima, Yasuo
AU  - Kojima Y
FAU - Hayashi, Mikiko
AU  - Hayashi M
FAU - Honda, Sachiko
AU  - Honda S
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - China
TA  - Asian J Androl
JT  - Asian journal of andrology
JID - 100942132
SB  - IM
MH  - Adult
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Klinefelter Syndrome/genetics/*physiopathology
MH  - Male
MH  - *Mosaicism
MH  - Pregnancy
MH  - Pregnancy Outcome
MH  - *Sperm Injections, Intracytoplasmic
EDAT- 2005/05/18 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/05/18 09:00
PHST- 2005/05/18 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/05/18 09:00 [entrez]
AID - 10.1111/j.1745-7262.2005.00023.x [doi]
PST - ppublish
SO  - Asian J Androl. 2005 Jun;7(2):217-20. doi: 10.1111/j.1745-7262.2005.00023.x.