PMID- 15898356
OWN - NLM
STAT- MEDLINE
DCOM- 20050906
LR  - 20191109
IS  - 0921-9668 (Print)
IS  - 0921-9668 (Linking)
VI  - 60
IP  - 1
DP  - 2005 Mar
TI  - Antimutagenic properties of green tea.
PG  - 25-9
AB  - In this work biological effects of two common kinds of green tea (Chinese 
      Gunpowder and Japanese Sencha) were analyzed using three independent tests of 
      antimutagenicity: 1) the Ames test with Salmonella typhimurium TA98, 2) 
      cytogenetic analysis of peripheral blood lymphocytes (CAPL), and 3) test with 
      Saccharomyces cerevisiae D7. Tea extracts were allowed to be antimutagenic based 
      on their ability to inhibit the mutagenic effect of standard mutagens. Amounts of 
      (-)catechin and (-)catechin gallate in tea extracts were determined by high 
      performance liquid chromatography on reversed phase (RP-HPLC). Antioxidant 
      capacity was found using total radical-trapping antioxidant parameter (TRAP) 
      method. Extracts from Gunpowder and Sencha exhibited high antimutagenic activity 
      in the Ames test (24.7+/-3.7% and 34.1+/-2, 1% of inhibition without metabolic 
      activation; 74.9+/-1.7% and 62.7+/-4.3% of inhibition with metabolic activation, 
      respectively) as well as in S. cerevisiae D7 test (Gunpowder: 62.7+/-5.7% of Trp 
      convertants inhibition and 52.6+/-5.3% of Ilv revertants inhibition; Sencha: 
      45.6+/-4.2% of Trp convertants inhibition, 50.0+/-4.8% of Ilv revertants 
      inhibition). In the CAPL method reduced number of abberant cells as well as 
      decreased number of chromosome breaks was observed using both green tea extracts. 
      Antioxidant capacity and antimutagenicity of green tea extracts was higher than 
      activity of tea catechins and flavonoids.
FAU - Bunkova, R
AU  - Bunkova R
AD  - Faculty of Chemistry, Brno University of Technology, Purkyova 118, 612 00 Brno, 
      Czech Republic.
FAU - Marova, I
AU  - Marova I
FAU - Nemec, M
AU  - Nemec M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Plant Foods Hum Nutr
JT  - Plant foods for human nutrition (Dordrecht, Netherlands)
JID - 8803554
RN  - 0 (Antimutagenic Agents)
RN  - 0 (Biogenic Amines)
RN  - 0 (Mutagens)
RN  - 0 (Plant Extracts)
RN  - 0 (Tea)
RN  - 8R1V1STN48 (Catechin)
SB  - IM
MH  - Antimutagenic Agents/*analysis
MH  - Biogenic Amines/analysis
MH  - Catechin/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - Mutagenicity Tests/*methods
MH  - Mutagens/analysis
MH  - Oxidation-Reduction
MH  - Plant Extracts/*analysis
MH  - Tea/*chemistry
EDAT- 2005/05/19 09:00
MHDA- 2005/09/07 09:00
CRDT- 2005/05/19 09:00
PHST- 2005/05/19 09:00 [pubmed]
PHST- 2005/09/07 09:00 [medline]
PHST- 2005/05/19 09:00 [entrez]
AID - 10.1007/s11130-005-2539-7 [doi]
PST - ppublish
SO  - Plant Foods Hum Nutr. 2005 Mar;60(1):25-9. doi: 10.1007/s11130-005-2539-7.