PMID- 15899040
OWN - NLM
STAT- MEDLINE
DCOM- 20060119
LR  - 20181201
IS  - 1478-6362 (Electronic)
IS  - 1478-6354 (Print)
IS  - 1478-6354 (Linking)
VI  - 7
IP  - 3
DP  - 2005
TI  - Synovial microparticles from arthritic patients modulate chemokine and cytokine 
      release by synoviocytes.
PG  - R536-44
AB  - Synovial fluid from patients with various arthritides contains procoagulant, 
      cell-derived microparticles. Here we studied whether synovial microparticles 
      modulate the release of chemokines and cytokines by fibroblast-like synoviocytes 
      (FLS). Microparticles, isolated from the synovial fluid of rheumatoid arthritis 
      (RA) and arthritis control (AC) patients (n = 8 and n = 3, respectively), were 
      identified and quantified by flow cytometry. Simultaneously, arthroscopically 
      guided synovial biopsies were taken from the same knee joint as the synovial 
      fluid. FLS were isolated, cultured, and incubated for 24 hours in the absence or 
      presence of autologous microparticles. Subsequently, cell-free culture 
      supernatants were collected and concentrations of monocyte chemoattractant 
      protein-1 (MCP-1), IL-6, IL-8, granulocyte/macrophage colony-stimulating factor 
      (GM-CSF), vascular endothelial growth factor (VEGF) and intracellular adhesion 
      molecule-1 (ICAM-1) were determined. Results were consistent with previous 
      observations: synovial fluid from all RA as well as AC patients contained 
      microparticles of monocytic and granulocytic origin. Incubation with autologous 
      microparticles increased the levels of MCP-1, IL-8 and RANTES in 6 of 11 cultures 
      of FLS, and IL-6, ICAM-1 and VEGF in 10 cultures. Total numbers of microparticles 
      were correlated with the IL-8 (r = 0.91, P < 0.0001) and MCP-1 concentrations (r 
      = 0.81, P < 0.0001), as did the numbers of granulocyte-derived microparticles (r 
      = 0.89, P < 0.0001 and r = 0.93, P < 0.0001, respectively). In contrast, GM-CSF 
      levels were decreased. These results demonstrate that microparticles might 
      modulate the release of chemokines and cytokines by FLS and might therefore have 
      a function in synovial inflammation and angiogenesis.
FAU - Berckmans, Rene J
AU  - Berckmans RJ
AD  - Department of Clinical Chemistry, Academic Medical Center, University of 
      Amsterdam, Amsterdam, The Netherlands. r.j.berckmans@amc.nl
FAU - Nieuwland, Rienk
AU  - Nieuwland R
FAU - Kraan, Maarten C
AU  - Kraan MC
FAU - Schaap, Marianne C L
AU  - Schaap MC
FAU - Pots, Desiree
AU  - Pots D
FAU - Smeets, Tom J M
AU  - Smeets TJ
FAU - Sturk, Augueste
AU  - Sturk A
FAU - Tak, Paul P
AU  - Tak PP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20050301
PL  - England
TA  - Arthritis Res Ther
JT  - Arthritis research & therapy
JID - 101154438
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Arthritis, Rheumatoid/*metabolism/pathology
MH  - Cells, Cultured
MH  - Chemokines/*metabolism
MH  - Cytokines/*metabolism
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Synovial Fluid/cytology/*metabolism/physiology
PMC - PMC1174949
EDAT- 2005/05/19 09:00
MHDA- 2006/01/20 09:00
PMCR- 2005/03/01
CRDT- 2005/05/19 09:00
PHST- 2004/10/13 00:00 [received]
PHST- 2005/01/26 00:00 [revised]
PHST- 2005/02/02 00:00 [accepted]
PHST- 2005/05/19 09:00 [pubmed]
PHST- 2006/01/20 09:00 [medline]
PHST- 2005/05/19 09:00 [entrez]
PHST- 2005/03/01 00:00 [pmc-release]
AID - ar1706 [pii]
AID - 10.1186/ar1706 [doi]
PST - ppublish
SO  - Arthritis Res Ther. 2005;7(3):R536-44. doi: 10.1186/ar1706. Epub 2005 Mar 1.