PMID- 15899532
OWN - NLM
STAT- MEDLINE
DCOM- 20050916
LR  - 20191210
IS  - 0168-1656 (Print)
IS  - 0168-1656 (Linking)
VI  - 118
IP  - 1
DP  - 2005 Jul 21
TI  - Microbial adaptation to biodegradation of tert-butyl alcohol in a sequencing 
      batch reactor.
PG  - 45-53
AB  - This study demonstrates the utility of the sequencing batch reactor (SBR) to 
      adapt microorganisms towards biological removal of tert-butyl alcohol (TBA). The 
      reactor was inoculated with activated sludge and fed with TBA as the sole carbon 
      source. Start-of-cycle TBA concentrations were initially set at 100 mgL(-1) with 
      a cycle time of 24 h and a volumetric exchange ratio of 50% to maintain a TBA 
      loading rate of not more than 100 mgL(-1)d(-1). Step increases in TBA loading 
      rates up to 600 mgL(-1)d(-1) were achieved by first raising the start-of-cycle 
      TBA concentration to 150 mgL(-1) on day 90 and subsequently by reducing the cycle 
      time from 24 to 12, 8 and 6h on days 100, 121 and 199, respectively. This 
      acclimation strategy favored the retention of increasingly higher densities of 
      well-adapted microbial populations in the reactor. The increases in TBA loading 
      produced better settling biomass and higher biomass concentrations with higher 
      specific TBA biodegradation rates. Effluent TBA concentrations were consistently 
      below the detection limit of 25 microgL(-1). The use of progressively shorter 
      cycle times created selection pressures that fostered the self-immobilization of 
      the reactor microorganisms into aerobic granules which first appeared on day 125. 
      Specific TBA biodegradation rates in the granules followed the Haldane model for 
      substrate inhibition, and peaked at 13.8 mgTBAgVSS(-1)h(-1) at a TBA 
      concentration of 300 mgL(-1). Denaturing gradient gel electrophoresis (DGGE) 
      analysis of PCR-amplified 16S rRNA genes from granules sampled between days 220 
      and 247 confirmed the existence of a highly stable microbial community with 
      members belonging to the alpha, beta and delta subdivisions of Proteobacteria and 
      the Cytophaga-Flavobacteria-Bacteroides (CFB) group.
FAU - Zhuang, Wei-Qin
AU  - Zhuang WQ
AD  - Environmental Engineering Research Centre, School of Civil and Environmental 
      Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 
      639798, Singapore.
FAU - Tay, Joo-Hwa
AU  - Tay JH
FAU - Yi, Shan
AU  - Yi S
FAU - Tay, Stephen Tiong-Lee
AU  - Tay ST
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Biotechnol
JT  - Journal of biotechnology
JID - 8411927
RN  - 0 (Sewage)
RN  - 0 (Water Pollutants, Chemical)
RN  - MD83SFE959 (tert-Butyl Alcohol)
SB  - IM
MH  - Adaptation, Physiological/physiology
MH  - Bacteria/*isolation & purification
MH  - *Bacterial Physiological Phenomena
MH  - Biodegradation, Environmental
MH  - Bioreactors/*microbiology
MH  - Cell Culture Techniques/instrumentation/*methods
MH  - Sewage/*microbiology
MH  - Water Pollutants, Chemical/pharmacokinetics
MH  - Water Purification/methods
MH  - tert-Butyl Alcohol/*pharmacokinetics
EDAT- 2005/05/19 09:00
MHDA- 2005/09/17 09:00
CRDT- 2005/05/19 09:00
PHST- 2004/10/06 00:00 [received]
PHST- 2005/02/17 00:00 [revised]
PHST- 2005/02/25 00:00 [accepted]
PHST- 2005/05/19 09:00 [pubmed]
PHST- 2005/09/17 09:00 [medline]
PHST- 2005/05/19 09:00 [entrez]
AID - S0168-1656(05)00139-2 [pii]
AID - 10.1016/j.jbiotec.2005.02.014 [doi]
PST - ppublish
SO  - J Biotechnol. 2005 Jul 21;118(1):45-53. doi: 10.1016/j.jbiotec.2005.02.014.