PMID- 15900488
OWN - NLM
STAT- MEDLINE
DCOM- 20050707
LR  - 20181113
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 57
IP  - 3-4
DP  - 2005 May
TI  - The HLA-G genotype is associated with IL-10 levels in activated PBMCs.
PG  - 172-81
AB  - Human leukocyte antigen (HLA)-G is an MHC class Ib molecule that is expressed at 
      the feto-maternal interface during pregnancy. However, recent results have also 
      shown that it may have important functions as an immuno-modulatory factor in 
      adult life. Differences in the pattern of alternative splicing and in the 
      stability of HLA-G mRNA transcripts have been associated with HLA-G 
      polymorphisms, especially a 14 bp deletion/insertion polymorphism in the 3' 
      untranslated region of the HLA-G gene. We have investigated the secretion of 
      HLA-G5/soluble HLA-G1 and interleukin-10 (IL-10) in lipopolysaccharide 
      (LPS)-activated peripheral blood mononuclear lymphocytes (PBMCs) in relation to 
      the HLA-G 14 bp genotype. No HLA-G5/sHLA-G1 could be detected in the 
      non-activated control PBMC culture media, and there were no significant 
      differences among the three HLA-G 14 bp genotypes regarding IL-10 concentrations. 
      In LPS-activated PBMC cultures, no significant differences among the three HLA-G 
      14 bp genotypes regarding HLA-G5/sHLA-G1 concentrations were observed. However, 
      this was in contrast to the IL-10 levels (P=0.0004, Kruskal-Wallis test). The 
      +14/+14 bp PBMC samples expressed higher levels of IL-10 when compared to the 
      -14/+14 bp genotype and the -14/-14 bp genotype. Interestingly, the IL-10 G/G 
      polymorphism at position -1082 was more frequent in the +14/+14 bp genotype 
      (P=0.024, chi2 test). These results support an autocrine loop between 
      HLA-G5/sHLA-G1 and IL-10 expression in activated PBMCs, which may result in 
      higher IL-10 levels in +14/+14 bp HLA-G genotypes.
FAU - Rizzo, Roberta
AU  - Rizzo R
AD  - Department of Experimental and Diagnostic Medicine, University of Ferrara, Via 
      Luigi Borsari 46, 44100 Ferrara, Italy. rbr@unife.it
FAU - Hviid, Thomas Vauvert F
AU  - Hviid TV
FAU - Stignani, Marina
AU  - Stignani M
FAU - Balboni, Alessandra
AU  - Balboni A
FAU - Grappa, Maria Teresa
AU  - Grappa MT
FAU - Melchiorri, Loredana
AU  - Melchiorri L
FAU - Baricordi, Olavio R
AU  - Baricordi OR
LA  - eng
PT  - Journal Article
DEP - 20050402
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (3' Untranslated Regions)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Lipopolysaccharides)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Adult
MH  - Exons
MH  - Female
MH  - Genetic Linkage
MH  - Genotype
MH  - HLA Antigens/blood/*genetics
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/blood/*genetics
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-10/*blood/genetics
MH  - Lipopolysaccharides/pharmacology
MH  - Lymphocyte Activation
MH  - Lymphocytes/drug effects/immunology
MH  - Male
MH  - Middle Aged
MH  - Polymorphism, Genetic
MH  - Polymorphism, Single Nucleotide
EDAT- 2005/05/19 09:00
MHDA- 2005/07/08 09:00
CRDT- 2005/05/19 09:00
PHST- 2005/01/31 00:00 [received]
PHST- 2005/02/03 00:00 [accepted]
PHST- 2005/05/19 09:00 [pubmed]
PHST- 2005/07/08 09:00 [medline]
PHST- 2005/05/19 09:00 [entrez]
AID - 10.1007/s00251-005-0788-0 [doi]
PST - ppublish
SO  - Immunogenetics. 2005 May;57(3-4):172-81. doi: 10.1007/s00251-005-0788-0. Epub 
      2005 Apr 2.