PMID- 15900585
OWN - NLM
STAT- MEDLINE
DCOM- 20051121
LR  - 20160303
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 117
IP  - 2
DP  - 2005 Nov 1
TI  - Genomewide loss of heterozygosity and its clinical associations in non small cell 
      lung cancer.
PG  - 241-7
AB  - We extensively allelotyped a panel of 71 microdissected primary surgically 
      resected non small cell lung cancer (NSCLC) tumors to identify chromosomal 
      regions that are likely to contain tumor suppressor genes (TSGs) or associated 
      with clinicopathologic and prognostic effects. Loss of heterozygosity (LOH) was 
      detected by genotyping of 177 microsatellite markers and correlation of LOH with 
      clinicopathologic parameters and prognosis was analyzed. Twenty markers showed an 
      LOH frequency greater than 48%, and 8 of them (2p23.3, 2p24.3, 2q35, 6p22.2, 
      7p14.3, 7p22.2, 17q24.3 and 21q22.3) were novel in NSCLC. The high LOH regions 
      were confirmed by further aligning continuous LOH regions from another set of 24 
      NSCLC tissues and defining 7 minimal deletion regions ranging from 1.29 to 12.26 
      cM. The aberrations of 8 markers showed a significant correlation with alteration 
      of p16 and Rb proteins, suggesting the gene(s) located in the chromosomal loss 
      that may interact with p16/Rb pathway. In addition, markers specifically 
      associated with smoking, histology types and tumor stages were identified and the 
      linked candidate TSGs were suggested. For example, marker D1S1612 closely linked 
      with Mig-6 gene was associated with smoking patients, squamous cell carcinoma 
      patients and late-stage patients. Furthermore, 3 markers, D2S2968, D6S2439 and 
      D7S1818, were significantly associated with poor prognosis of NSCLC patients 
      using both univariate and multivariate Cox's regression analyses (p = 0.035, 
      0.022 and 0.006, respectively). These markers can potentially be used for early 
      lung cancer detection, outcome measurement and the positional cloning of new TSGs 
      whose loss of function contributes to NSCLC tumorigenesis.
FAU - Tseng, Ruo-Chia
AU  - Tseng RC
AD  - Department of Life Sciences, National Taiwan Normal University, Taipei, Taiwan.
FAU - Chang, Jer-Wei
AU  - Chang JW
FAU - Hsien, Feng-Jen
AU  - Hsien FJ
FAU - Chang, Ya-Hui
AU  - Chang YH
FAU - Hsiao, Chin-Fu
AU  - Hsiao CF
FAU - Chen, Jung-Ta
AU  - Chen JT
FAU - Chen, Chih-Yi
AU  - Chen CY
FAU - Jou, Yuh-Shan
AU  - Jou YS
FAU - Wang, Yi-Ching
AU  - Wang YC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Aged
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/mortality/pathology
MH  - Chromosome Mapping
MH  - Female
MH  - Genetic Markers
MH  - *Genome, Human
MH  - Humans
MH  - *Loss of Heterozygosity
MH  - Lung Neoplasms/*genetics/mortality/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Survival Analysis
EDAT- 2005/05/19 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/05/19 09:00
PHST- 2005/05/19 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/05/19 09:00 [entrez]
AID - 10.1002/ijc.21178 [doi]
PST - ppublish
SO  - Int J Cancer. 2005 Nov 1;117(2):241-7. doi: 10.1002/ijc.21178.