PMID- 15900681
OWN - NLM
STAT- MEDLINE
DCOM- 20050627
LR  - 20061115
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 64
IP  - 1
DP  - 2005 Apr 6
TI  - Detection of ostreid herpesvirus-1 (OsHV-1) by PCR using a rapid and simple 
      method of DNA extraction from oyster larvae.
PG  - 1-4
AB  - A DNA extraction procedure was developed for the detection of ostreid 
      herpesvirus-1 (OsHV-1) using the polymerase chain reaction (PCR) in oyster 
      larvae. The DNA extraction procedure developed was tested on 8 larval samples. 
      Abnormal nuclei with characteristic features associated with OsHV-1 infections 
      were only observed in samples in which the viral DNA was detected by PCR. A 
      previously described competitive PCR method was applied to detect inhibition 
      during PCR reactions. The results show that the method can be used on small 
      amounts of oyster larvae (3 mg) for the detection of OsHV-1 DNA by PCR.
FAU - Batista, Frederico M
AU  - Batista FM
AD  - Instituto Nacional de Investigacao Agraria e das Pescas (INIAPIIPIMAR), CRIPSul, 
      Av. 5 de Outubro, 8700-305 Olhao, Portugal.
FAU - Taris, Nicolas
AU  - Taris N
FAU - Boudry, Pierre
AU  - Boudry P
FAU - Renault, Tristan
AU  - Renault T
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
RN  - 0 (DNA Primers)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Aquaculture/methods
MH  - DNA/*isolation & purification
MH  - DNA Primers
MH  - Electrophoresis, Agar Gel
MH  - Herpesviridae/*genetics
MH  - Larva/virology
MH  - Ostreidae/*virology
MH  - Polymerase Chain Reaction/*methods
EDAT- 2005/05/20 09:00
MHDA- 2005/06/28 09:00
CRDT- 2005/05/20 09:00
PHST- 2005/05/20 09:00 [pubmed]
PHST- 2005/06/28 09:00 [medline]
PHST- 2005/05/20 09:00 [entrez]
AID - 10.3354/dao064001 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2005 Apr 6;64(1):1-4. doi: 10.3354/dao064001.