PMID- 15902721
OWN - NLM
STAT- MEDLINE
DCOM- 20050628
LR  - 20190430
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 11
IP  - 19
DP  - 2005 May 21
TI  - Presence and integration of HBV DNA in mouse oocytes.
PG  - 2869-73
AB  - AIM: Hepatitis B is a worldwide public health problem. To explore the feasibility 
      of hepatitis B virus (HBV) vertical transmission via oocytes, the presence and 
      integration of HBV DNA in mouse oocytes were studied. METHODS: Genomic DNA was 
      isolated and metaphases were prepared, respectively from mouse oocytes cocultured 
      with pBR322-HBV DNA plasmids. PCR, Southern blot, dot hybridization and 
      fluorescence in situ hybridization (FISH) were performed to explore the existence 
      and integration of HBV DNA in oocytes. RESULTS: PCR detected positive bands in 
      the tested samples, and then Southern blot revealed clear hybridization signals 
      in PCR products. Final washing solutions were collected for dot hybridization and 
      no signal for HBV DNA was observed, which excluded the possibility that 
      contamination of washing solutions gave rise to positive results of PCR and 
      Southern blot. FISH demonstrated that 36 of 1,000 metaphases presented positive 
      signals. CONCLUSION: HBV DNA sequences are able to pass through the zona and 
      oolemma to enter into oocytes and to integrate into their chromosomes. HBV DNA 
      sequences might be brought into embryo via oocytes as vectors when they are 
      fertilized with normal spermatozoa.
FAU - Huang, Tian-Hua
AU  - Huang TH
AD  - Research Center of Reproductive Medicine, Shantou University Medical College, 22 
      Xinling Road, Shantou 515041, Guangdong Province, China. thhuang@stu.edu.cn
FAU - Zhang, Qing-Jian
AU  - Zhang QJ
FAU - Xie, Qing-Dong
AU  - Xie QD
FAU - Zeng, Li-Ping
AU  - Zeng LP
FAU - Zeng, Xi-Fan
AU  - Zeng XF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - DNA, Viral/physiology
MH  - Female
MH  - Hepatitis B/*transmission/*virology
MH  - Hepatitis B virus/*genetics/*growth & development
MH  - Infectious Disease Transmission, Vertical
MH  - Mice
MH  - Oocytes/*virology
MH  - Virus Integration
PMC - PMC4305652
EDAT- 2005/05/20 09:00
MHDA- 2005/06/29 09:00
PMCR- 2005/05/21
CRDT- 2005/05/20 09:00
PHST- 2005/05/20 09:00 [pubmed]
PHST- 2005/06/29 09:00 [medline]
PHST- 2005/05/20 09:00 [entrez]
PHST- 2005/05/21 00:00 [pmc-release]
AID - 10.3748/wjg.v11.i19.2869 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2005 May 21;11(19):2869-73. doi: 10.3748/wjg.v11.i19.2869.