PMID- 15902982
OWN - NLM
STAT- MEDLINE
DCOM- 20050901
LR  - 20190513
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 29
IP  - 2
DP  - 2005 Mar
TI  - A new GC-MS method for the determination of five amphetamines in human hair.
PG  - 135-9
AB  - A new gas chromatography-mass spectrometry method for the simultaneous 
      identification and quantitation of amphetamine (AP), methamphetamine (MA), 
      3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), 
      and 3,4-methylenedioxyethamphetamine (MDEA) in hair is proposed. Hair was 
      hydrolyzed in 1 M NaOH at 40 degrees C, subjected to extraction with 4:1 (v/v) 
      methylene chloride/isopropanol, and derivatized with pentafluoropropionic 
      anhydride (PFPA) and ethyl acetate. Calibration curves for the five analytes were 
      constructed over the concentration range 0.5-25.0 ng/mg, using their 
      pentadeuterated analogues as internal standards. The limits of detection and 
      quantitation obtained were 0.045 and 0.151 ng/mg for AP; 0.014 and 0.048 ng/mg 
      for MA; 0.013 and 0.043 ng/mg for MDA; 0.017 and 0.057 ng/mg for MDMA; and 0.007 
      and 0.023 ng/mg for MDEA. The accuracy of the method was found to be in the range 
      +/- 9%, and the coefficients of variation were less than 8%. Overall, 24 hair 
      specimens tested positive for one or more amphetamines, with average 
      concentrations of 0.88 ng/mg for AP, 10.14 ng/mg for MA, 1.30 ng/mg for MDA, and 
      8.87 ng/mg for MDMA. Only one specimen tested positive for MDEA with a 
      concentration of 0.84 ng/mg.
FAU - Villamor, J L
AU  - Villamor JL
AD  - Institute of Legal Medicine, Forensic Toxicology Service, Faculty of Medicine, 
      University of Santiago de Compostela, Spain.
FAU - Bermejo, A M
AU  - Bermejo AM
FAU - Fernandez, P
AU  - Fernandez P
FAU - Tabernero, M J
AU  - Tabernero MJ
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Amphetamines)
RN  - 0 (Fluorocarbons)
RN  - 0LFE7U11O5 (pentafluoropropionic anhydride)
SB  - IM
MH  - Amphetamines/*analysis
MH  - Fluorocarbons
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Hair/*chemistry
MH  - Humans
MH  - Reproducibility of Results
EDAT- 2005/05/21 09:00
MHDA- 2005/09/02 09:00
CRDT- 2005/05/21 09:00
PHST- 2005/05/21 09:00 [pubmed]
PHST- 2005/09/02 09:00 [medline]
PHST- 2005/05/21 09:00 [entrez]
AID - 10.1093/jat/29.2.135 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2005 Mar;29(2):135-9. doi: 10.1093/jat/29.2.135.