PMID- 15904965
OWN - NLM
STAT- MEDLINE
DCOM- 20070719
LR  - 20220331
IS  - 1532-2742 (Electronic)
IS  - 0163-4453 (Linking)
VI  - 52
IP  - 2
DP  - 2006 Feb
TI  - Genotypic diversity and epidemiology of high-level gentamicin resistant 
      Enterococcus in a Chinese hospital.
PG  - 124-30
AB  - OBJECTIVE: To investigate the antibiotics resistance of Enterococcus, the 
      aminoglycoside-modifying enzymes (AME) and homology of high-level gentamicin 
      resistant (HLGR) Enterococcus in clinical specimens for the implementation of 
      effective infection control measures. METHODS: The resistance of 13 antimicrobial 
      agents was determined by Kirby-Bauer (K-B) or agar dilution method. And the HLGR 
      and high-level streptomycin resistant (HLSR) isolates were screened by agar 
      screen. Production of beta-lactamases was tested by the nitrocefin disc method. 
      The aminoglycoside-modifying enzyme genes were detected by polymerase chain 
      reaction (PCR). Pulsed-field gel electrophoresis (PFGE) was used to analyze the 
      homology of HLGR isolates from in-patients. RESULTS: No isolates resistant to 
      linezolid, vancomycin and teicoplanin were found. Ampicillin-resistant isolates 
      did not produce beta-lactamases and 68 HLGR isolates were screened at the rate of 
      64.2%. The positive rate of aac(6')-Ie-aph(2'')-Ia was 86.8% and 3 isolates had 
      the new AME gene designated aph(2'')-Ie mostly similar to aph(2'')-Id. Among 51 
      HLGR isolates from in-patients, PFGE grouped 17 Enterococcus faecalis (E. 
      faecalis) isolates into 4 clusters (A-D), and 33 Enterococcus faecium (E. 
      faecium) isolates into 8 clusters (A-H), of which the A cluster is the main. 
      CONCLUSIONS: HLGR has become the important antibiotic resistance pathogen causing 
      nocosomial infection. And the aac(6')-Ie-aph(2'')-Ia gene was the main 
      aminoglycoside-modifying enzyme gene leading to HLGR.
FAU - Qu, Ting-Ting
AU  - Qu TT
AD  - Department of Infectious Diseases, First Affiliated Hospital, College of 
      Medicine, Zhejiang University, No. 79, Qing Chun Road, Hangzhou, Zhejiang 310003, 
      China. quxuerong@21cn.com
FAU - Chen, Ya-Gang
AU  - Chen YG
FAU - Yu, Yun-Song
AU  - Yu YS
FAU - Wei, Ze-Qing
AU  - Wei ZQ
FAU - Zhou, Zhi-Hui
AU  - Zhou ZH
FAU - Li, Lan-Juan
AU  - Li LJ
LA  - eng
SI  - GENBANK/AY677166
PT  - Journal Article
PL  - England
TA  - J Infect
JT  - The Journal of infection
JID - 7908424
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (Gentamicins)
RN  - EC 2.- (6'-aminoglycoside acetyltransferase-2''-aminoglycoside 
      phosphotransferase)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.7.1.- (APH(2'')-Ic protein, Enterococcus faecalis)
RN  - EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor))
RN  - EC 2.7.1.- (aminoglycoside 2''-phosphotransferase)
SB  - IM
MH  - Acetyltransferases/genetics
MH  - Anti-Infective Agents/*pharmacology
MH  - Bacterial Proteins/genetics
MH  - China/epidemiology
MH  - DNA Primers/chemistry
MH  - Electrophoresis, Gel, Pulsed-Field/methods
MH  - Enterococcus faecalis/*drug effects/genetics
MH  - Enterococcus faecium/*drug effects/genetics
MH  - Genetic Variation/genetics
MH  - Genotype
MH  - Gentamicins/*pharmacology
MH  - Gram-Positive Bacterial Infections/*epidemiology/genetics/*microbiology
MH  - Humans
MH  - Microbial Sensitivity Tests
MH  - Molecular Sequence Data
MH  - Phosphotransferases (Alcohol Group Acceptor)/genetics
MH  - Phylogeny
MH  - Polymerase Chain Reaction
EDAT- 2005/05/21 09:00
MHDA- 2007/07/20 09:00
CRDT- 2005/05/21 09:00
PHST- 2004/12/06 00:00 [received]
PHST- 2005/02/28 00:00 [accepted]
PHST- 2005/05/21 09:00 [pubmed]
PHST- 2007/07/20 09:00 [medline]
PHST- 2005/05/21 09:00 [entrez]
AID - S0163-4453(05)00069-1 [pii]
AID - 10.1016/j.jinf.2005.02.030 [doi]
PST - ppublish
SO  - J Infect. 2006 Feb;52(2):124-30. doi: 10.1016/j.jinf.2005.02.030.