PMID- 15905690
OWN - NLM
STAT- MEDLINE
DCOM- 20050829
LR  - 20191210
IS  - 1052-9551 (Print)
IS  - 1052-9551 (Linking)
VI  - 14
IP  - 2
DP  - 2005 Jun
TI  - Fluorescence in situ hybridization for the detection of t(X;18)(p11.2;q11.2) in a 
      synovial sarcoma tissue microarray using a breakapart-style probe.
PG  - 77-82
AB  - Synovial sarcomas (SSs) account for 5% of soft tissue tumors and carry a balanced 
      translocation t(X;18)(p11.2;q11.2), detectable in over 90% of cases. This 
      translocation brings together portions of two genes: SYT and SSX. Detecting 
      interruption of the SYT gene on chromosome 18 would be useful as a diagnostic 
      tool. We describe a scoring method to detect disruption of SYT with breakapart 
      probe fluorescence in situ hybridization (FISH) and the application of this 
      method for identification of SS within a sarcoma tissue microarray. After 
      optimization, SYT disruption was identified in 22 of 23 (96%) of known SS tumor 
      samples but was not in 23 of 23 (100%) of non-SS sarcoma samples. Ten of 11 (91%) 
      blinded test SS tumor samples were also correctly identified. For comparison, 
      commercially available FISH and chromogenic in situ hybridization (CISH) probes 
      were tested. The commercial FISH probes identified SYT disruption in 81% of the 
      SS tumor samples but in none of the non-SS samples. The CISH probes produced 
      signals too weak to interpret. The use of breakapart FISH probes is a relatively 
      quick procedure for detection of synovial sarcoma translocations and can be 
      applied to archival specimens in tissue microarrays.
FAU - Terry, Jefferson
AU  - Terry J
AD  - Genetic Pathology Evaluation Centre, British Columbia Cancer Agency, Vancouver, 
      British Columbia, Canada.
FAU - Barry, Todd S
AU  - Barry TS
FAU - Horsman, Douglas E
AU  - Horsman DE
FAU - Hsu, Forrest D
AU  - Hsu FD
FAU - Gown, Allen M
AU  - Gown AM
FAU - Huntsman, David G
AU  - Huntsman DG
FAU - Nielsen, Torsten O
AU  - Nielsen TO
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
RN  - 0 (DNA, Complementary)
RN  - 0 (Nucleic Acid Probes)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (SS18 protein, human)
SB  - IM
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Chromosomes, Human, Pair 18/*genetics
MH  - DNA, Complementary/chemistry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Nucleic Acid Probes
MH  - Proto-Oncogene Proteins/*genetics
MH  - Repressor Proteins/*genetics
MH  - Sarcoma, Synovial/*diagnosis/genetics
MH  - Sequence Deletion
MH  - Soft Tissue Neoplasms/*diagnosis/genetics
MH  - *Tissue Array Analysis
MH  - Translocation, Genetic
EDAT- 2005/05/21 09:00
MHDA- 2005/08/30 09:00
CRDT- 2005/05/21 09:00
PHST- 2005/05/21 09:00 [pubmed]
PHST- 2005/08/30 09:00 [medline]
PHST- 2005/05/21 09:00 [entrez]
AID - 00019606-200506000-00004 [pii]
AID - 10.1097/01.pas.0000155021.80213.c9 [doi]
PST - ppublish
SO  - Diagn Mol Pathol. 2005 Jun;14(2):77-82. doi: 10.1097/01.pas.0000155021.80213.c9.