PMID- 15905879
OWN - NLM
STAT- MEDLINE
DCOM- 20051110
LR  - 20161124
IS  - 1350-9047 (Print)
IS  - 1350-9047 (Linking)
VI  - 12
IP  - 10
DP  - 2005 Oct
TI  - Transcriptional upregulation of PUMA modulates endoplasmic reticulum calcium pool 
      depletion-induced apoptosis via Bax activation.
PG  - 1310-8
AB  - PUMA, a key mediator of p53-induced apoptosis, is a BH3-only domain proapoptotic 
      protein that localizes to mitochondria and interacts with antiapoptotic Bcl-2 and 
      Bcl-X(L). Recent evidence implicates Bax to be an important mediator of 
      PUMA-activated apoptotic signals. We have previously demonstrated that Bax 
      deficiency significantly affects thapsigargin (TG)-mediated endoplasmic reticulum 
      calcium pool depletion-induced apoptosis. We now present evidence that TG 
      upregulates PUMA expression and that although Bax-deficient cells exhibit 
      resistance to TG, Bax deficiency does not attenuate TG upregulation of PUMA 
      expression. Furthermore, TG transcriptionally upregulates PUMA expression in a 
      p53-independent manner and that PUMA-deficient cells are more resistant to 
      undergo TG-induced apoptosis than the PUMA-proficient counterparts. Thus, our 
      results demonstrate that TG engages PUMA and Bax for full transduction of 
      apoptotic signals and both PUMA and Bax appear to exist in the same TG-activated 
      apoptotic pathway in which PUMA may reside upstream of Bax.
FAU - Luo, X
AU  - Luo X
AD  - Department of Pharmacology, State University of New York, Upstate Medical 
      University, Syracuse, NY 13210, USA.
FAU - He, Q
AU  - He Q
FAU - Huang, Y
AU  - Huang Y
FAU - Sheikh, M S
AU  - Sheikh MS
LA  - eng
GR  - CA86945/CA/NCI NIH HHS/United States
GR  - DK062136/DK/NIDDK NIH HHS/United States
GR  - DK067271/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Cell Death Differ
JT  - Cell death and differentiation
JID - 9437445
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (BAX protein, human)
RN  - 0 (BBC3 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Tumor Protein p73)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 67526-95-8 (Thapsigargin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Apoptosis/drug effects/*physiology/radiation effects
MH  - Apoptosis Regulatory Proteins
MH  - Base Sequence
MH  - Calcium/deficiency/*metabolism
MH  - Cell Line, Tumor
MH  - DNA-Binding Proteins/deficiency/metabolism
MH  - Endoplasmic Reticulum/*metabolism
MH  - Genes, Tumor Suppressor
MH  - HCT116 Cells
MH  - Humans
MH  - Male
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/deficiency/metabolism
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/deficiency/*metabolism
MH  - Thapsigargin/pharmacology
MH  - Transcription, Genetic/drug effects
MH  - Tumor Protein p73
MH  - Tumor Suppressor Protein p53/*metabolism
MH  - Tumor Suppressor Proteins
MH  - Up-Regulation/drug effects
MH  - bcl-2-Associated X Protein
EDAT- 2005/05/21 09:00
MHDA- 2005/11/11 09:00
CRDT- 2005/05/21 09:00
PHST- 2005/05/21 09:00 [pubmed]
PHST- 2005/11/11 09:00 [medline]
PHST- 2005/05/21 09:00 [entrez]
AID - 4401659 [pii]
AID - 10.1038/sj.cdd.4401659 [doi]
PST - ppublish
SO  - Cell Death Differ. 2005 Oct;12(10):1310-8. doi: 10.1038/sj.cdd.4401659.