PMID- 15906520
OWN - NLM
STAT- MEDLINE
DCOM- 20110310
LR  - 20050523
IS  - 1673-0860 (Print)
IS  - 1673-0860 (Linking)
VI  - 40
IP  - 1
DP  - 2005 Jan
TI  - [Microsatellite instability and loss of heterozygosity of fragile histidine triad 
      gene in laryngeal squamous cell carcinoma].
PG  - 45-8
AB  - OBJECTIVE: To investigate the loss of heterozygosity (LOH) and microsatellite 
      instability (MSI) of fragile histidine triad (FHIT) gene in laryngeal squamous 
      cell carcinoma (LSCC). METHODS: Using polymerase chain reaction-simple sequence 
      length polymorphism (SSLP) -silver staining technique, 41 samples of LSCC were 
      detected for LOH and MSI with microsatellite sites D3S1234 and D3S1300. RESULTS: 
      LOH was found in 44.4% (16/36) and 36.4% (12/33) of informative cases at D3S1234 
      and D3S1300 respectively, while MSI at the two loci was found in 19.4% (7/36) and 
      24.2% (8/33) of informative cases respectively. In 41 cases of LSCC, 38 cases 
      were informative at either locus. 52.6% (20/38) of cases had LOH while 28.9% 
      (11/38) had MSI. The rate of LOH was related to the tumor TNM stage, pathological 
      grade, lymph node metastasis and tumor relapse (P < 0.05). The rate of MSI was 
      related to the tumor lymph node metastasis (P < 0.05 ). CONCLUSION: The 
      frequencies of LOH and MSI in the two microsatellite sites, D3S1234 and D3S1300, 
      of FHIT gene might play a role in carcinogenesis and development of LSCC and 
      might provide some new approaches for early gene detection for LSCC.
FAU - Yin, De-Tao
AU  - Yin DT
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated 
      Hospital of Zhengzhou University, Zhengzhou 450052, China. detaoyin@zzu.edu.cn
FAU - Dong, Ming-Min
AU  - Dong MM
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
JT  - Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of 
      otorhinolaryngology head and neck surgery
JID - 101247574
RN  - 0 (Neoplasm Proteins)
RN  - 0 (fragile histidine triad protein)
RN  - EC 3.6.- (Acid Anhydride Hydrolases)
SB  - IM
MH  - Acid Anhydride Hydrolases/*genetics
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/*genetics/pathology
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - *Loss of Heterozygosity
MH  - Male
MH  - *Microsatellite Instability
MH  - Middle Aged
MH  - Neoplasm Proteins/*genetics
EDAT- 2005/05/24 09:00
MHDA- 2011/03/11 06:00
CRDT- 2005/05/24 09:00
PHST- 2005/05/24 09:00 [pubmed]
PHST- 2011/03/11 06:00 [medline]
PHST- 2005/05/24 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2005 Jan;40(1):45-8.