PMID- 15910245
OWN - NLM
STAT- MEDLINE
DCOM- 20050819
LR  - 20161124
IS  - 1547-3287 (Print)
IS  - 1547-3287 (Linking)
VI  - 14
IP  - 2
DP  - 2005 Apr
TI  - Differential gene expression profiling of CD34+ CD133+ umbilical cord blood 
      hematopoietic stem progenitor cells.
PG  - 188-98
AB  - Umbilical cord blood (CB)-derived primitive hematopoietic stem progenitor cells 
      (HSPC) are a promising source for stem cell-based gene therapy due to the reduced 
      incidence and severity of graftversus- host disease (GVHD) after human leukocyte 
      antigen (HLA)-disparate CB transplantation. Cell-surface markers such as CD34 and 
      CD133 have been used in combination to enrich primitive HSPC for research and 
      clinical applications. To understand the molecular characteristics of the CB 
      HSPC, we compared the global gene expression of freshly isolated CB CD34+ CD133+ 
      cells with their progenies using a cDNA microarray containing 22,000 human cDNA 
      clones printed on a single chip. A total of 139 genes were differentially 
      expressed between CB HSPC and their progenies. These transcripts included a 
      number of known genes that might play roles in key functions of CB HSPC as well 
      as many genes of unknown function. Among the genes showing the greatest 
      differential expression levels in HSPC were: psoriasin 1, CRHBP, HDAC3, MLLT3, 
      HBEX2, SPINK2, c-kit, H2BFQ, CD133, HHEX, TCF4, ALDH1A1, and FHL1. These data 
      provide more information on the molecular phenotype of CB HSPC and may lead to 
      the identification of new genes critical to stem cell function.
FAU - He, Xianghui
AU  - He X
AD  - Gene Therapy Group, Department of Microbiology and Immunology, University of 
      Arizona, Tucson, AZ 85724, USA.
FAU - Gonzalez, Veronica
AU  - Gonzalez V
FAU - Tsang, Amy
AU  - Tsang A
FAU - Thompson, Jennifer
AU  - Thompson J
FAU - Tsang, Tom C
AU  - Tsang TC
FAU - Harris, David T
AU  - Harris DT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (AC133 Antigen)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, CD34)
RN  - 0 (DNA, Complementary)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Glycoproteins)
RN  - 0 (PROM1 protein, human)
RN  - 0 (Peptides)
RN  - 113849-17-5 (9-(4-chlorophenyl) 7,7-dimethyl-5,8-nonadienoic acid methyl ester)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - AC133 Antigen
MH  - Antigens, CD
MH  - Antigens, CD34/*biosynthesis
MH  - Cell Proliferation
MH  - DNA, Complementary/metabolism
MH  - Fatty Acids, Unsaturated
MH  - Fetal Blood/*cytology
MH  - Flow Cytometry
MH  - Gene Expression Profiling/*methods
MH  - *Gene Expression Regulation
MH  - Glycoproteins/*biosynthesis
MH  - Graft vs Host Disease/metabolism
MH  - Hematopoietic Stem Cells/*cytology
MH  - Humans
MH  - Nucleic Acid Hybridization
MH  - Oligonucleotide Array Sequence Analysis
MH  - Peptides
MH  - Phenotype
MH  - RNA/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stem Cells/*cytology
MH  - Time Factors
MH  - Umbilical Cord/*cytology
EDAT- 2005/05/25 09:00
MHDA- 2005/08/20 09:00
CRDT- 2005/05/25 09:00
PHST- 2005/05/25 09:00 [pubmed]
PHST- 2005/08/20 09:00 [medline]
PHST- 2005/05/25 09:00 [entrez]
AID - 10.1089/scd.2005.14.188 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2005 Apr;14(2):188-98. doi: 10.1089/scd.2005.14.188.